由低抗凝肝素制得低分子肝素的分子量与抗血栓作用的关系

Correlation of molecular weight of low molecular weight heparin from low anticoagulant activity heparin with antithrombotic activity

以生产商品肝素的副产品—低抗凝肝素为原料,通过亚硝酸控制解聚制得低分子肝素粗品。将平均分子量分别为7700,5800和4300d的粗品经凝胶层析纯化制得了分子量为7100,5300和4000d的低分子肝素,测定了其理化指标和抗凝效价。以从刺激开始到血栓形成这段堵塞时间(OT)为药效指标,研究了其抗血栓形成作用,OT值分别为37.97±2.84,63.03±3.42,28.72±1.75min。分子量为5300d的低分子肝素抗血栓形成作用最强。

From low anticoagulant activity heparin, a sideproduct accompanying commercial heparin, three re-fined products of low molecular weight heparin(IMWH) were prepared and their effects on an-tithrombotic activity were evaluated in experimentalrat thrombus model by measuring the time (known asocclusion time) from the beginning of electric stimu-lation to the formation of thrombus in the commoncarotid artery. The results showed that the values ofocclusion time for saline, commercial heparin andthree products with molecular weight 7100, 5300 and4000 d were 17.76±2.51, 24.21±1.89, 37.97±2.84, 63.03±3.42 and 28.72±1.75 min re-spectively. The antithrombotic activity of the LMWHwith molecular weight 5300 d was the strongest.