热毒宁对脂多糖小鼠急性肺损伤和血红素加氧酶-1活性的影响

Effects of Reduning injection on acute lung injury and heme oygenase-1 activity in LPS-induced mice

目的观察热毒宁注射液（reduning，RDN）对脂多糖（LPS）诱导小鼠的急性肺损伤（acute lung injury，ALI）保护作用，探讨其作用是否与血红素加氧酶-1（HO-1）有关。方法将48只雄性ICR小鼠平均随机分为Control组、RDN组、LPS组、LPS＋地塞米松（DEX）组、LPS＋RDN组、LPs＋RDN＋ZnPP（HO-1特异性抑制剂，锌原卟啉）组，每组8只。采用气管内滴注LPS（5mg/kg）诱导ALI；造模后24h，采集静脉血、取肺组织和支气管肺泡灌洗液（BALF），观察各组肺组织病理学改变，测量肺湿／干重比（W／D），BALF中的细胞总数和血清LDH活性、肺组织HO-1活性和HO-1蛋白表达量。结果与LPS组相比，LPS＋RDN组大鼠肺组织损伤较轻，肺W／D、BALF中细胞总数和血清LDH活性显著降低，HO-1的活性和蛋白表达量显著升高，差异均有统计学意义（P〈0．05）。与LPS＋RDN组相比，LPS＋RDN＋ZnPP组大鼠肺W／D、BALF中细胞总数、血清LDH活性显著升高，HO-1的活性和蛋白表达量减低，均差异有统计学意义（P〈0．05）。结论RDN对LPS诱导的ALI有保护作用，其保护作用与HO-1表达和活性增加有关。

Objective To investigate the effect of Reduning （RDN） injection on acute lung injury （ALI） and heme oxygenase-l（HO-1） in LPS-. induced mice. Methods 48 male ICR mice were randomly divided into six groups： control group, LPS group, RDN group, LPS＋RDN group, LPS＋DEX group and LPS＋RDN＋ZnPP group. Mice were intratracheal instillation with LPS （5mg/kg） to induce ALI. After LPS administration for 24 h, bronchoalveolar lavage fluid （BALF） and lung tissue were collected for the histopathological examinations. Lung wet/dry （W/D） ratio, total cell counts, the content of LDH, lung tissue HO-lactivity and HO-1 protein expression were examined. Results Compared with LPS group, lung W/D ratio, total cell counts and content of LDH were significantly decreased （P 〈0.05） while lung tissueHO-1 activity and HO-1 protein expression were significantly increased （P 〈0.05） in LPS＋RDN group. The pathological changes of the lung were also significantly attenuated in LPS ＋ RDN group and LPS ＋ DEX group as compared to LPS group. ZnPP abolished the protective effects of RDN on LPS-induced ALL Conclusion RDN has protective effect on LPS-induced ALI, and its protective effect is related to the increase of HO-1 expression and activity.