Foxp3基因多态性与妊娠期高血压的相关性

Correlation between Foxp3 gene polymorphism and hypertensive disorder complicating pregnancy

目的通过对叉状头/翅膀状螺旋转录因子3(Foxp3)多态性基因位点的序列分析,探讨Foxp3基因多态性分布及基因型变异对妊娠期高血压(HDCP)发病的易感性,以阐明其在DHCP发生中的分子机制。方法选取186例妊娠期高血压患者,设为病例组;另选取同期251例本院住院的正常孕妇,设为对照组。分析两组Foxp3-6054、924位点的基因多态性与HDCP的相关性。结果两组研究对象年龄比较,差异无统计学意义(P>0.05),两组高血压家族史、孕周、收缩压(SBP)、舒张压(DBP)比较,差异具有统计学意义(P<0.05)。Foxp3-924基因多态性检测中两组基因频率比较,差异无统计学意义(P>0.05)。病例组患者中T/T基因型及T等位基因频率明显少于对照组,差异具有统计学意义(P<0.05);病例组A/A基因型频率明显多于对照组,差异具有统计学意义(P<0.05)。Logistic回归分析提示AT+TT型可减少HDCP的发生风险(OR=0.367,95%CI为0.218,0.617,P<0.05),而联合基因型AA+AT可增加罹患HDCP的风险(OR=2.518,95%CI为1.654,3.832,P<0.05)。结论 Foxp3-6054位点基因型及等位基因频率在HDCP组与正常妊娠组间存在显著性差异,Foxp3-6054位点多态性可能参与HDCP的发生,而Foxp3-924基因多态性可能与HDCP无关。

Objective To investigate the distribution of forkhead/winged helix transcription factor 3（Foxp3） gene polymorphism and the susceptibility of genotype variation to hypertensive disorder complicating pregnancy（HDCP） in order to clarify the molecular mechanism of pregnancy induced hypertension. Methods There were 186 patients with pregnancy induced hypertension as case group, and concurrent 251 normal pregnant women as control group. The correlation between gene polymorphism and HDCP of Foxp3-6054 and 924 loci and HDCP in two groups was analyzed. Results Both groups had no statistically significant difference in mean age（P〈0.05）. Both groups had statistically significant difference in family history of hypertension, gestational age, systolic blood pressure（SBP）, diastolic blood pressure（DBP）（P〈0.05）. Both groups had no statistically significant difference in gene frequency in Foxp3-924 gene polymorphism detection（P〉0.05）. The case group had obviously less T/T genotype and T allele frequency than the control group, and the difference was statistically significant（P〈0.05）. The case group had obviously more A/A genotype frequency than the control group, and the difference was statistically significant（P〈0.05）. Logistic regression analysis suggested that AT ＋ TT could reduce the risk of HDCP（OR=0.367, 95%CI as 0.218, 0.617, P〈0.05）, and combined genotype AA ＋ AT could increase the risk of developing HDCP（OR=2.518, 95%CI as 1.654, 3.832, P〈0.05）. Conclusion Foxp3-6054 locus genotype and allele frequency were significantly different between HDCP group and normal pregnancy group. Foxp3-6054 polymorphism might be involved in the occurrence of HDCP, and Foxp3-924 gene polymorphism might be related to HDCP Irrelevant.