γ-氨基丁酸对鳜摄食和食欲的影响(英)

EFFECTS OF γ-AMINOBUTYRIC ACID ON FOOD INTAKE AND APPETITE IN MANDARIN FISH SINIPERCA CHUATSI

为阐明γ-氨基丁酸(GABA)对鳜(Siniperca chuatsi)摄食和食欲的影响,对鳜脑室注射生理盐水和不同剂量的GABA(50、125、500和2000μg)。结果显示,注射125μg GABA组的鳜在2h内摄食量显著升高。实时荧光定量PCR(RT-q PCR)结果显示,注射125μg GABA 0.5h后,鳜鱼脑中Ag RP和NPY m RNA表达量上调,CART和POMC m RNA表达量下调,都和鳜摄食量增加相一致。相比于对照组,注射GABA后Leptin-R的m RNA表达量在0.5h和2h都有显著下降。这些结果表明GABA可能通过leptin的信号通路来影响食欲,进而影响摄食量。研究结果可以为GABA在水产饲料中的应用提供理论依据。

γ-Aminobutyric acid（GABA） has been established as an important regulator of food intake in mammals, but its role in fish remains scant. The aim of this study was to investigate the effects of GABA on food intake in mandarin fish（Siniperca chuatsi）. Fish received intracerebroventricular（ICV） injection of saline and different doses of GABA（50, 125, 500 and 2000 μg）. Food intake increased significantly in 125 μg GABA group within 2 h. The RT-q PCR data demonstrated that up-regulation of NPY and Ag RP, and downregulation of CART and POMC were consistent with higher food intake of mandarin fish. The m RNA expression of Leptin-R decreased significantly at all the four doses of GABA at 0.5 h and 2 h compared to the saline injected fish. These results indicated that GABA could affect the appetite by regulating leptin signaling pathway and thus resulting in alteration of food intake. Our findings could provide theory basis for GABA application in aquaculture feeds.