枸杞种质资源遗传多样性的iPBS分析

Genetic diversity analysis of wolfberry germplasm using iPBS makers

采用引物结合位点扩增(iPBS)分子标记技术对34份枸杞种质资源进行遗传多样性分析.从83条iPBS引物中筛选出11条引物分别对34份枸杞种质基因组DNA进行扩增,共检测到91条清晰谱带,其中,多态性条带89条,多态性比率为97.8%,平均多态信息含量为0.46.采用POPGENE软件计算34份种质的平均有效等位基因数为1.570,平均Nei's基因多样性指数为0.327,平均Shannon信息指数为0.489,表明34份种质具有丰富的遗传多样性.采用NTSYS-pc软件计算得到34份种质间的遗传相似系数为0.56~0.93,非加权组平均法(UPGMA)聚类分析结果表明,遗传相似系数为0.65时,可将34份种质分成5大类群,反映出栽培品种与野生种质遗传差异大,亲缘关系较远.iPBS分子标记可有效用于枸杞种质资源遗传多样性分析.

The genetic diversity among 34 wolfberry germplasm resources were analyzed using an inter-primer binding site(iPBS)molecular marker.Eleven primers were screened from 83 iPBS primers to amplify the genomic DNA of the tested materials.A total of91 scorable bands were detected,of which 89 bands were polymorphic(97.8%).The average value of polymorphic information content was 0.46.By POPGENE software,average value of effective number of alleles,Nei's gene diversity and Shannon's information index was 1.570,0.327 and 0.489,respectively,indicating that a high level of genetic diversity among 34 wolfberry germplasm.The similarity coefficient among 34 germplasm ranged from 0.56 to 0.93 by NTSYS-pc software.Based on unweighted pair group method with arithmetic means(UPGMA) cluster analysis,34 tested germplasm could be divided into 5 groups when genetic similarity was0.65.In conclusion,the genetic relationship of cultivars tested were not close to wild germplasm resources.The iPBS molecular markers can be effectively used for genetic diversity analysis of wolfberry germplasm resources and provide theoretical and technical support for scientific management and utilization of wolfberry germplasm resources.