基于生物碱类成分优选黄连药材的加工方法

Optimization of processing methods for Coptidis Rhizoma based on composition of alkaloids

目的:探索黄连药材在产地加工过程中不同的干燥方法对其生物碱类成分的影响,以优化和建立适宜的干燥加工方法。方法:采用高效液相色谱(HPLC)法同时测定不同加工方法的黄连药材样品中7种生物碱类成分的含量,并采用主成分分析(PCA)对其进行综合评价。结果:不同加工方法对黄连中生物碱类成分的含量有一定影响,自然干燥综合质量较好。结论:建议黄连在加工时采用低温、不断翻动干燥的方法,为优选黄连适宜的产地加工方法提供了基础研究资料。

OBJECTIVE To explore the effect of different processing methods of alkaloids in Coptidis Rhizoma to optimize and establish appropriate drying methods. METHODS Seven alkaloids from Coptidis Rhizoma in different processing methods were simultaneously determined by HPLC. In addition, the principal component analysis （PCA） was carried out for a compre- hensive evaluation. RESULTS The different processing methods of Coptidis Rhizoma showed certain influence on 7 alkaloids, and the shade dried samples had better quality. CONCLUSION The method of drying by constantly turning at low tempera- tures is recommended as the optimum original processing method, which provides a scientific basis for suitable processing of Coptidis Rhizoma.