超高效液相色谱法测定大鼠血浆中α-萜品烯醇含量及其药代动力学研究

Determination and Pharmacokinetic Study of α-Terpineol in Rat Plasma by Ultra-high Performance Liquid Chromatography

目的:用超高效液相色谱法(Ultra Performance Liquid Chromatography,UPLC)测定大鼠血浆中α-萜品烯醇含量,研究α-萜品烯醇在大鼠体内药物代谢情况。方法:SD大鼠灌胃给药,在给药后不同时间点取血,用UPLC检测不同时间点血样中α-萜品烯醇含量,绘制药时曲线,运用DAS.2软件计算药动学参数。结果:α-萜品烯醇的药代动力学最佳模型为一室模型,其主要药动学参数为AUC_((0→t))为(635.935±322.888)mg/(L·h),AUC_((0→∞))为(1 127.278±518.188)mg/(L·h),t_(1/2)z为(4.787±2.049)h,T_(max)为(0.5±0.329)h,C_(max)为(176.268±82.236)mg·L^(-1)。结论:该方法快速、简便、准确,可用于大鼠血浆中α-萜品烯醇的含量测定。

Objective： The aim of this study is to develop the quantitative method of α- terpineol in rat plasma by Ul- tra Performance Liquid Chromatography（UPLC） and investigate its pharmacokinetic for rats. Methods： The concentrations of paeonifiorin and albifiorin in plasma at different time after dosing were determined with UPLC method. The pharmaco- kinetic parameters were calculated by DAS. 2 pharmacokinetic program and the drug - time curve was drawn. Results ： The optimal pharmacokinetic compartmental model of α- terpineol was one compartmental model. The pharmacokintic param- eters of α-terpineol were as follows ： AUC（0→t） （635. 935 ± 22. 888） mg/（ L h）, AUC（0→∞） （ 1 127. 278 ±518. 188） mg/（L h）, t1/2z （4.787±2.049） h, Tmax（0. 5 ±0.329） h, Cmax（176.268±82.236） mg L-1. Conclusion： It is proved that the method with UPLC is rapid, simple and accurate, which can be used for α-terpineol in rat plasma.