基于酶联免疫法检测盐酸克伦特罗的微流控系统

Microfludic system for clenbuterol detection based on enzyme-linked immunosobent assay

基于微流控技术和酶联免疫法的原理建立动物性食品中盐酸克伦特罗残留的检测技术,确保食品安全。设计制作的微流控芯片结合搭建的微流控酶联免疫实验平台,可以实现对动物性食品中盐酸克伦特罗的现场、简便、快速的免疫反应和检测。结果表明:通过在芯片上包被盐酸克伦特罗抗原,由实验平台自动完成进样、清洗过程,30 min内即可实现对盐酸克伦特罗的快速检测。标准品检测范围为(0.10~8.10)ng/m L,加标回收率在95.5%~107.3%之间。使用微流控盐酸克伦特罗残留检测芯片系统,具有快速、自动化程度高、灵敏度高、成本低、方法学性能良好等优势,特别适用于非专业人士进行盐酸克伦特罗残留的现场、快速检测。

A method was established for the clenbuterol detection in animal products based on the microfludic technology and enzyme-linked immunosobent assay in order to ensure food safety. By coupling with an experimental platform, the chip could provide a simple, easy to use for rapid detection of the clenbuterol in animal products. Due to envelop the clenbuterol antigen in the microfuldic chip, and complete the process of washing and sampling automatically through experimental platform, the detection could be achieved within 30 min. The detection range of sample concentration was between 0.10 ng/m L and 8.10 ng/m L, the recoveries of clenbuterol in swine urine were in the range of 95.5% to 107.3% The method for the detection of clenbuterol based on the microfludic is simple, rapid, highly sensitive and low cast, and has a good performance of methodology, which is particularly suitable for on-site, rapid detection by non-professionals.