二次回归正交旋转组合设计优化大肥蘑菇液体培养基

Optimization of Agaricus bitorquis Liquid Culture Medium by Quadratic Regression Orthogonal Rotary

为优化大肥蘑菇液体培养基,通过单因子试验确定大肥蘑菇最佳碳源(葡萄糖)、最佳氮源(蛋白胨)及矿物质的适宜浓度(用量)范围,采用二次回归旋转组合设计研究3个参数对大肥蘑菇菌丝生物量的影响,建立数学模型,以获得适宜的配方组合。结果表明,葡萄糖浓度、蛋白胨浓度对大肥蘑菇菌丝体生物量的影响达极显著水平,矿物质添加剂用量达显著水平。最优培养基参数为葡萄糖浓度33.26 g/L、蛋白胨浓度4.24 g/L、矿物质添加剂1.82 mL/L,在该参数组合下,28℃振荡培养8 d,菌丝干重可达16.44 g/L,且经反复试验验证可行。

In order to optimize the liquid medium ofAgaricus bitorquis, the best density（dosage） and appropriate concentration （dosage） range of main factors is determined by the single factor experiment and main factors including glucose, peptone and mineral additive. Further, the influence of three parameters on mycelia biomass is carried out using quadratic orthogonal rotation combination design, established the mathematical model, obtained the appropriate combination formula. The experimental result shows that the effects of glucose concentration, peptone concentration on mycelium biomass reached extremely significant level, and mineral additives to significant level. The optimal medium parameters which glucose is 33.26 g/L, peptone is 4.24 g/L, mineral additives is 1.82 mL/L. Experiment is performed with the optimal parameters which 28 ℃ shaking culture 8 d, mycelial dry weight reach to 16.44 g/L. Prediction result is reliable and the model is validated.