响应面分析优化蒽酮硫酸法测定葡萄叶片中可溶性糖的含量

Determination of Soluble Sugar from Grape Leaves by Optimizing of Anthrone-Sulfuric Acid Method by Response Surface Methodology

测定葡萄叶片中的可溶性糖,对葡萄叶片可溶性糖提取条件进行了比较;利用响应面法(RSM)对测定可溶性糖含量的蒽酮-硫酸显色方法参数进行优化,并得到回归模型。结果表明,葡萄叶片可溶性糖提取的最佳提取剂为水,采用21.9%乙酸锌和10.6%亚铁氰化钾可以最大限度的去除共提物干扰,确定检测波长为625nm。用0.2 mg/mL蒽酮-硫酸试剂12 mL,在沸水浴中加热12 min。在此条件下测定的可溶性糖平均含量为2.54%~6.93%,精密度实验RSD为0.68%~0.81%,平均加标回收率99.4%,RSD=2.7%(n=3)。

An anthrone-sulfuric acid method for the extraction of soluble sugar from grape leaves is optimized using response surface methodology （RSM）. The result indicates the optimum extraction agent of soluble sugar glucose is water, adding 21.9% zinc acetate and 10.6% potassium ferrocyanide could not interfere with co-extract extremely, and detection wavelength is 625 nm. In addition, we suggest 12 mL of 0.2 mg/mL anthrone-sulfuric acid agent should be added and perform water-bathing for 12 min. on the basis of the conditions, the content of soluble sugar is found in the range of 2.54~6.93%, the relative standard deviation （RSD） ranged from 0.68% to 0.81%, and the average recovery is 99.4% （RSD=2.7%, n=3）.