肠愈灌肠方治疗溃疡性结肠炎大鼠实验研究

Effect of Changyu Enema Prescription in Treatment of Rats with Ulcerative Colitis:an Experimental Study

目的观察肠愈灌肠方治疗溃疡性结肠炎(ulcerative colitis,UC)的疗效,并探讨其作用机制。方法将64只SPF大鼠按随机数字表法分为正常对照组、模型组、美沙拉嗪组、肠愈灌肠方组。使用2,4,6-三硝基苯磺酸结肠灌注法复制UC大鼠模型,在实验第12天观察各组大鼠的疾病活动指数(disease activity index,DAI);实验结束后采用ELISA法检测大鼠结肠黏膜组织转化生长因子-β_1(transforming growth factor-β_1,TGF-β_1)、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)的含量。结果与模型组比较,肠愈灌肠组、美沙拉嗪组大鼠DAI评分明显降低(P<0.05),血清TGF-β_1显著上升(P<0.05),TNF-α显著下降(P<0.05)。结论肠愈灌肠方治疗UC的疗效确切,其作用机制可能与降低血清促炎因子,促进抑炎因子表达有关。

Objective To investigate the effect of Changyu Enema Prescription in the treatment of ulcera- tive colitis （UC） and related mechanism of action. Methods A total of 64 specific pathogen-free rats were randomly divided into normal control group, model group, mesalazine group, and Changyu Enema Pre- scription group, using a random number table. Colon perfusion with 2,4,6-trinitrobenzenesulfonic acid was performed to establish a rat model of UC, and disease activity index （DAD was observed on day 12 of the experiment. After the experiment ended, ELISA was used to measure the levels of transforming growth factor-β1 （TGF-β1） and tumor necrosis factor-α （TNF-α） in the colonic mucosa. Results Compared with the.model group, the Changyu Enema Prescription group and the mesalazine group had a significant reduction in DAI, a sig- nificant increase in serum TGF-β1 , and a significant reduction in TNF-a （all P〈0.05）. Conclusion Changyu En- ema Prescription has a marked effect in the treatment of UC, possibly by reducing the serum levels of proinflam- matory factors and promoting the expression of anti-inflammatory cytokines.