摘要
目的建立从草苁蓉和肉苁蓉中提取总苷的含量测定方法,并对草苁蓉和肉苁蓉中的总苷含量进行测定。方法选择红景天苷作为对照品,使用香草醛-高氯酸溶液进行显色反应,用分光光度法在523nm波长下对草苁蓉和肉苁蓉中总苷的含量进行测定。结果红景天苷在0.05133~0.2567mg·ml^(-1)线性关系良好,回归方程Y=1.611X+0.0573(r=0.9992),草苁蓉的平均回收率为99.68%,RSD 1.68%,肉苁蓉的平均回收率为101.18%,RSD为0.94%。结论该方法精密度较高、简便、稳定性好,可用于草苁蓉和肉苁蓉中总苷含量的测定。
Objective This quantitative method is established of total saponins in Boschniakia rossica and Herba cistanches and the content of the total saponins is determinated. Methods Salidroside was selected as reference substance,color reaction was used with vanillin and perchloric acid. Then the content of total saponins in Boschniakia rossica and Herba cistanches was determined at 523 nm by spectrophotometry. Results The salidroside curve was linear over the range of 0. 05133 ~ 0. 2567 mg·ml^-1 with the correlation of 0. 9992,and the linear regression equation was Y = 1. 611 X ＋ 0. 0573. For Boschniakia rossica,the average recovery of salidroside was 99. 68% with RSD of 1. 68%（ n = 6）; and for Herba cistanches,the average recovery of salidroside was 101. 18% with RSD of 0. 94%（ n = 6）. Conclusion The method is accurate,simple,reliable. So it can be used to determine the content of total saponins in Boschniakia rossica and Herba cistanches.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2017年第10期2336-2339,共4页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(31100254
81102672)
中央级公益性科研院所基本科研业务费专项资金(ZZ2016013)
吉林省教育厅项目(吉教科合字[2014]第399号)
关键词
草苁蓉
肉苁蓉
总苷
含量测定
分光光度法
红景天苷
Boschniakia rossica
Herba cistanches
Total saponins
Determination
Spectrophotometry
Salidroside
