贝加尔唐松草内生真菌的分离及抗氧化活性研究

Studies on isolation and antioxidant activities of endophytic fungi from Thalictrum baicalense Turcz.

目的分离和鉴定贝加尔唐松草内生真菌,并检测其次级代谢产物的抗氧化活性。方法采用组织分离法从其根、茎、叶和种子中分离内生真菌,根据形态进行初步鉴定;用DPPH自由基清除法评价贝加尔唐松草内生真菌发酵产物的抗氧化活性。结果从贝加尔唐松草中共分离得到18株内生真菌,它们分属于曲霉属、顶孢霉属、镰刀菌属、香柱菌属、链格孢菌属、匍柄霉属。菌株发酵液的乙酸乙酯部位普遍具有抗氧化活性,其中Y-3对DPPH自由基清除活性较强,达到91.04%,仅次于Vc。结论贝加尔唐松草内生真菌的次级代谢产物具有抗氧化活性,是一种较好的天然抗氧化剂。

Objective Isolation and identification of endophytic fungi from Thalictrum baicalense Turcz.,then studies on antioxidant activities of their secondary metabolites. Methods The endophytic fungi were isolated by using tissue isolation method from the roots,stems,leaves and seeds of the plant and identified preliminarily based on morphological characteristics. Antioxidant activities were evaluated by DPPH radical scavenging. Results Totally 18 fungi were obtained,identified and classified to be Aspergillus,Acremonium,Fusarium,Epichloe,Alternaria,Stemphylium. The ethyl acetate extracts of the fermentation from various fungi exhibited antioxidant activities. Strain Y-3 showed the best DPPH radical scavenging activity with clearance rate of 91. 04%,which is closing to that of Vc. Conclusion The secondary metabolites of endophytic fungi from Thalictrum baicalense Turcz. had powerful antioxidant activities,which are effective and natural antioxidants.