摘要
目的应用高速离心分配色谱法(FCPC)分离纯化葛根中的大豆苷。方法以乙酸乙酯-正丁醇-乙醇-水(5:1:0.5:5)作为两相溶剂系统,上相为流动相,下相为固定相,转速1400 r/min,流速3.0 m L/min,检测波长254 nm,采用ascending模式进行分离制备,化合物的结构由质谱及核磁共振谱鉴定。结果从1.0 g葛根粗提物中分离得到16.3 mg大豆苷,纯度经HPLC分析为96.5%。结论该方法适合于葛根中的大豆苷的高效分离制备。
Objective To isolate and purify daidzin from the roots of Pueraria lobata by fast centrifugal partition chromatography（ FCPC）. Methods The experiment was performed with a two phase solvent system composed of ethyl acetate-n-butanol-ethanol-water（ 5： 1： 0. 5： 5,v/v）. The upper phase as mobile phase in ascending mode was operated at a rotation speed of 1400 r/min and the flow rate of 3. 0 m L/min with the detection wave length at 254 nm. The compound was analyzed by high performance liquid chromatography（ HPLC）,and elucidated by comprehensive NMR and ESI-MS analysis. Results A total 16. 3 mg of daidzin with the purity about 96. 5% was successfully obtained by FCPC from 1. 0 g of the crude extract of Pueraria lobata. Conclusion The FCPC was a simple,robust and efficient method for the preparation of daidzin from Pueraria lobata.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2017年第10期2386-2388,共3页
Lishizhen Medicine and Materia Medica Research
基金
上海市国际合作项目(12120701400)
上海市自然科学基金(09ZR1431100)
国家"重大新药创制"科技重大专项(2009ZX09301-007)
上海市中药现代化专项资助项目(10DZ1971300)
上海市科委西部开发科技合作项目(10495801000)
关键词
高速离心分配色谱
葛根
大豆苷
Fast centrifugal partition chromatography
Pueraria lobata
Daidzin
