摘要
荧光技术已发展成为分子生物学、细胞生物学、分析化学、环境检测和临床诊断的有力工具，具有灵敏度高，选择性好，时空分辨率高，设备简单廉价等突出优势。荧光探针通常由荧光基团和识别基团组成。识别基团可以选择性感知环境参数，也可以识别小分子、离子、酶、蛋白、核酸等。识别事件如何产生荧光响应，需要研究荧光发射的基本规律。当染料吸收光到达激发态后，其能量可以荧光形式释放，也可以被内转换、电子转移、能量转移、或系间穿越等过程耗散。识别事件通过对上述激发态能量释放途径的影响，从而产生荧光变化，实现对客体的荧光响应和检测。荧光探针的设计和使用涉及多学科，发展荧光探针对现代毕命科学和精准医学意义尤为重要。
Fluorescent probes including chemosensors and chemodosimeters are widely used in molecular biology, cellular biology, immunobiology, analytical chemistry and environmental detection because of their high sensitivity and selectivity, together with the advantages of spatial and temporal resolution, and the low cost and This special issue contains reviews or articles related to fluorescent probes, we hope that the readers will lave a deep understanding of the current development in this area, and get benefit from the papers for their ＇uture research.
出处
《应用化学》
CSCD
北大核心
2017年第12期1343-1343,共1页
Chinese Journal of Applied Chemistry
