寨卡病毒感染者13例病毒核酸检测结果分析

Zika virus nucleic acid detection of biological samples from 13 Chinese infected in a foreign country

目的分析广东省江门市13例寨卡病毒感染者生物样品病毒核酸结果。方法采用描述性方法对2016年2—4月江门市报告的寨卡病毒感染者进行流行病学分析。采用荧光定量逆转录聚合酶链反应(qRTPCR)方法检测研究对象的血液、尿液和唾液寨卡病毒核酸。结果 2016年2—4月江门市共确诊10例寨卡病毒病病例和发现3例寨卡病毒隐性感染者,均自委内瑞拉输入。13例感染者中,男性6例,女性7例;最小年龄5岁,最大年龄46岁,中位数是19岁,≤14岁儿童占46.15%(6/13)。寨卡病毒核酸阳性检出持续时间,血液最短0 d,最长4 d,中位数0 d;尿液最短2 d,最长32 d,中位数5.5 d;唾液最短0 d,最长10 d,中位数3.5 d;尿液样品比血液样品病毒核酸阳性持续时间长(P<0.01);唾液样品比血液样品病毒核酸阳性持续时间长(P<0.05)。结论寨卡病毒感染者血液、尿液和唾液3种生物样品病毒核酸阳性持续时间不一致。

Objective To analyze the results of Zika virus nucleic acid detection of biological samples from 13 Chi- nese returning to Jiangmen city after viral infection in a foreign country. Methods Medical records were extracted and blood, urine and saliva samples were collected for all Zika virus infected patients reported in Jiangmen city of Guangdong province between February and April 2016. All collected samples were detected for Zika virus nucleic acid with fluores- cent quantitative real time reverse transcription-polymerase chain reaction （qRT-PCR）. Results Totally 10 overt and 3 covert Zika virus infections were reported in Jiangmen city during the 3-month period and all the reported cases were Chi- nese returning to China from Venezuela. Of the 13 cases,6 were male and 7 were female;the minimum age of the cases was 5 years and the maximum was 46 years,with a median age of 19 years;46. 15% （6） of the cases were children un- der 14 years old. The positive duration of Zika virus nucleic acid detection ranged 0 -4 days for blood,2 -32 days for urine, and 0 - 4 days for saliva samples, with the medians of 0,5.5,3.5 days, respectively; both the positive duration of urine and saliva samples were significantly longer than that of blood （ P 〈 0. 01 and P 〈 0. 05 ）. Conclusion The positive duration of viral nucleic acid iS different for blood,urine, and saliva samples from Zika virus infected people.