解毒扶正散对非小细胞肺癌免疫微环境的影响

Effect of Jiedu Fuzheng San on Tumor Immune Microenvironment of Non-Small Cell Lung Cancer

目的探讨非小细胞肺癌患者经中医方剂解毒扶正治疗后肿瘤免疫微环境相关细胞因子的变化,探索中医药治疗非小细胞肺癌的作用机制。方法将60非小细胞肺癌患者按随机数字表法分为治疗组和对照组:治疗组30例,采用化疗+解毒扶正散治疗;对照组30例,采用单纯化疗方案,共2个疗程,每个疗程28d。采用ELISA检测2组患者外周血中干扰素(IFN-γ)、白细胞介素2(IL-2)、转化生长因子-β(TGF-β)的水平,采用流式细胞仪检测2组患者CD4+CD25+调节性T细胞的比例。结果治疗组和对照组性别、年龄比较差异无统计学意义(P>0.05)。治疗组较对照组血浆中IFN-γ、IL-2水平提高,CD4+CD25+调节性T细胞比例提高,TGF-β水平降低,差异均有统计学意义(均P<0.05)。结论解毒扶正散对非小细胞肺癌患者机体免疫功能调节具有一定的作用,可改善非小细胞肺癌患者肿瘤免疫微环境,为今后开展大样本、前瞻性临床研究提供了新的佐证。

Objective To investigate the changes in cytokines in tumor immune microenvironment of non-small cell lung cancer(NSCLC)after treatment with Jiedu Fuzheng San,and to explore the mechanism of action of traditional Chinese medicine for NSCLC.Methods Sixty NSCLC patients were randomly given chemotherapy alone(control group,n=30)or in combination with 2 courses of 28-day Jiedu Fuzheng San treatment(treatment group,n=30).The levels of interferon-γ(IFN-γ),interleukin-2(IL-2)and transforming growth factor-β(TGF-β)in peripheral blood were measured by ELISA.The proportion of CD4+CD25+T cells was analyzed by flow cytometry.Results There were no significant differences in gender and age between the two groups(P>0.05).Compared with control group,the levels of IFN-γand IL-2 and proportion of CD4+CD25+T cells increased but the levels of TGF-βdecreased in treatment group(P<0.05).Conclusion Jiedu Fuzheng San can regulate immune function and tumor microenvironment in patients with NSCLC.These results provide new evidence for prospective and controlled clinical trial.