清肺抑火中药干预大气PM_(2.5)对大鼠肺部炎症损伤的实验研究

Traditional Chinese medicine intervention effect on rats' pulmonary inflammation damage induced by PM_(2.5)

目的探讨中药清肺抑火中药对PM_(2.5)致大鼠肺部炎症损伤的干预作用。方法将42只健康成年Wistar雄性大鼠随机分为7组,分别为空白对照组、生理盐水对照组、干预对照组、染毒对照组、低剂量干预组、中剂量干预组和高剂量干预组。处理结束后,收集各组大鼠肺泡灌洗液(BALF),并测定BALF中生化指标和炎症因子水平的变化。结果与生理盐水对照组比较,染毒对照组BALF中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)的活力和总蛋白(TP)、白蛋白(ALB)、白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子(TNF-α)、超敏C反应蛋白(HS-CRP)含量均升高,差异有统计学意义(P<0.05)。低、中、高剂量干预组BALF中生化指标和炎症因子水平比染毒对照组水平降低,差异有统计学意义(P<0.05)。结论清肺抑火中药可能通过抗细胞毒性和抗炎症作用,减轻PM_(2.5)暴露对大鼠肺部产生的损伤。

Objective To explore the intervention effect of Qingfeiyihuo capsule on rats' pulmonary inflammation damage induced by fine particulate matter(PM_(2.5)). Methods Forty-two healthy adult male Wistar rats were randomly divided into seven groups: blank group: saline group,intervening control group,PM_(2.5)-exposure group,and intervening groups(low,moderate and high-dose). The levels of ACP,AKP,LDH,TP,ALB,IL-1β,IL-6,TNF-α and HS-CRP in brouchoalveolar lavage fluid(BALF) were analyzed. Results The levels of ACP,AKP,LDH,TP,ALB,IL-1β,IL-6,TNF-α and HS-CRP in the PM_(2.5)-exposure group were higher than those in the saline group,whose differences were statistically significant(P < 0. 05). The levels of ACP,AKP,LDH,TP,ALB,IL-1β,IL-6,TNF-α and HS-CRP in the intervening groups were lower than those in the PM_(2.5)-exposure group with statistical significances(P < 0. 05).Conclusion Qingfeiyihuo capsule may reduce the pulmonary inflammation damage induced by PM_(2.5).