脑血疏对大鼠脑出血的行为学及凝血功能的影响

Effects of Naoxueshu on the behavior and coagulation in the intracerebral hemorrhage in rats

目的研究脑血疏对大鼠脑出血(intracerebral hemorrhage,ICH)的行为学及凝血功能的影响。方法 21只大鼠通过注射Ⅳ型胶原酶建立ICH模型,采用数字表法随机分为:对照组、A组和B组,A、B组分别给予高浓度和低浓度脑血疏,神经功能评分采用Longa评分法、前肢踩空试验和12分评分法3种方法分别在脑出血后24、48、72 h进行神经功能评分和称体质量,72 h后采血进行凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)、纤维蛋白原(fibrinogen,Fib)、凝血酶时间(thrombin time,TT)、国际标准化比值(International Normalized Ratio,INR)检测。结果体质量比较显示:A组和B组的24 h与72 h体质量比较差异有统计学意义(P<0.05),B组和对照组的72 h体质量比较差异有统计学意义(P<0.05);Longa评分结果显示:B组的24、48和72 h Longa评分差异有统计学意义(P<0.05);凝血四项指标结果显示:B组和对照组的PT差异有统计学意义(P<0.05),A组和对照组的APTT差异有统计学意义(P<0.05),B组和对照组的APTT差异有统计学意义(P<0.05),B组和对照组的INR差异有统计学意义(P<0.05)。结论脑血疏可以改善脑出血急性期大鼠体质量下降以及神经功能评分,机制与其影响凝血功能有关。

Objective To study the protective effect of Naoxueshu in the intracerebral hemorrhage in rats and its effects on the coagulation.Methods Totally 21 rats were injected with type Ⅳ collagenase were randomly divided into 3 groups,group A,group B and the control group.Group A and B were respectively given high and low concentration of Naoxueshu.We recorded the weight and assessed neurological function before sacrificing the rats at 24 hours,48 hours,and 72 hours,and tested the coagulation parameters finally.Results The weight significantly increased at 24 h compared with that at the 72 h in group A and B（P0.05）.Compared with the control group,the weights significantly increased in group B at 72 h（P0.05）.The neurological function scores in group B were significantly decreased at 24 h,48 h and 72 h（P0.05）.Compared with the control group,the activated partial thromboplastin time（APTT） and International Normalized Ratio（INR） significantly decreased in group B（P0.05）.Conclusion Naoxueshu could improve the weights and the neurological defects in the acute phase of intracerebral hemorrhage in rats,the mechanism maybe associated with its coagulation function.