一贯煎及其加味对辐射损伤小鼠防护作用研究

Protective effect of Yiguanjian and its additives against radiation injury in mice

目的探讨一贯煎及其加味对辐射损伤小鼠的防护作用及其机制。方法以6 MV X直线加速器一次性全身照射小鼠造成辐射损伤,观察一贯煎及其加味对小鼠外周血象、外周血超氧化物歧化酶和谷胱甘肽过氧化物酶活性水平及免疫功能(胸腺、脾指数)、骨髓细胞计数、血浆与骨髓环磷酸腺苷水平等指标的变化,推测其对小鼠造血功能、免疫功能的影响及其可能机制。结果成功制备辐射损伤小鼠模型。一贯煎原方及其加味能升高辐射损伤小鼠外周血中白细胞计数、血小板计数、血红蛋白及骨髓细胞计数(P<0.05),提高胸腺、脾指数(P<0.05),升高骨髓环磷酸腺苷水平和谷胱甘肽过氧化物酶活性(P<0.05),各指标在原方组与加味组比较差异均无统计学意义(P>0.05)。结论一贯煎原方及其加味对小鼠辐射损伤有较好防护作用,能在一定程度上促进辐射损伤小鼠造血与免疫功能恢复,抗自由基损伤可能是其作用机制之一。

Objective To discuss the protective effect and mechanisms of Yiguanjian and its additives on mice with radiation injury. Methods Expose the mice to total body irradiation by 6 MV X linear accelerator to cause radiation injury,to observe the changes of Yiguanjian and its additives on indexes such as peripheral hemogram,superoxide dismutase（ SOD） and glutathione peroxidase（ GPX） activity levels,immune functions（ thymus coefficient and spleen coefficient）,bone marrow cells（ BMC）,cyclic adenosine monophosphate（ c AMP） levels in plasma and bone marrow,and infer their influence on hematopoietic function,immune function and the possible mechanisms. Results We built up the radiation injury mouse model successfully. Yiguanjian and its additives could increase white blood cells（ WBC）,hemoglobin（ Hb）,platelet（ PLT） of peripheral blood and BMC（ P〈0. 05）,rise the thymus coefficient and spleen coefficient（ P〈0. 05）,together with c AMP levels in bone marrow and GPX activity levels（ P〈0. 05）. The difference of the related indexes between the Yiguanjian group and the additive group was not obvious（ P〉0. 05）. Conclusion Yiguanjian and its additives have preferable protective effects on irradiated mice,they can promote the recovery of hematopoietic function and immune function of mice with radiation injury to a certain extent,anti-free radical injury may be one of the possible mechanisms.