摘要
研究pH值、温度、光照对芍药苷的影响以及其在生物样品中的稳定性。采用高效液相色谱法(high performance liquid chromatography,HPLC)测定芍药苷在不同化学条件下及生物样品中的浓度。结果发现,芍药苷的稳定性受强碱和温度影响明显,光照影响不明显。芍药苷在肝脏、肾脏、心脏等的组织匀浆液中相对稳定,而在尿液、胆汁和血浆中有降解趋势,且降低温度可减少芍药苷在生物样品中的降解。因此,生物样品的采集、处理和保存均应在低温下进行,在处理过程中要避免接触碱性环境,需将采集的生物样品冷冻保存。
The effects of pH, temperature and light conditions on paeoniflorin and its stability in vitro biological samples were in-vestigated. High performance liquid chromatography （HPLC） was used to determine the concentration of pachemicfl conditions and biological samples. The results show that the stability of paeoniflorin s significantly affected by strong a- kaliand temperature,the effect of light is not obvious. Paeoniflorin m the liver kidney heart and other tissue homogenate s sta-ble ,and has a tendency of degradation in urine , bile and plasma. Therefore, the collection, treatment and preservation of biologi-cal samples should be carried out at low temperatures.
出处
《中国科技论文》
北大核心
2017年第18期2092-2097,共6页
China Sciencepaper
基金
国家自然科学基金资助项目(81430095)
高等学校博士学科点专项科研基金资助项目(20132134110006)
天津市应用基础及前沿技术研究计划资助项目(16JCYBJC27900)
关键词
中药化学
芍药苷
生物样品
稳定性
高效液相
chemistry of Chinese materia medica
paeoniflorin
biological sample
stability
high performance phy
