Wnt2在奶牛胎盘发育过程中的表达与定位

Expression and localization of Wnt2 during placental development in dairy cows

胎盘发育异常是体细胞克隆失败的主要原因之一,但其机制知之甚少,而Wnt信号通路广泛参与细胞命运决定、胎儿发育和胎盘形成等生命过程。采用qRT-PCR和原位杂交等方法检测Wnt2在奶牛胎盘中的表达与定位,发现Wnt2主要富集在子叶中并定位于子叶滋养层细胞上。结果表明,Wnt2在奶牛胎盘发育过程中发挥着重要的调节功能。为解决奶牛胎盘发育异常的问题找到新的分子作用靶点。

The placenta dysplasia is one of the main causes for the failure of the somatic cell derstood. Wnt signaling pathway is widely involved in life processes such as cell fate determintal formation and so on. Real-time quantitative PCR and in situ hybridization were employed to detect the expression and localiza-tion of Wnt2 during bovine placenta and found that Wnt2 mRNA was more abundant in cotyledoncotyledon trophoblastic cells. These results indicated that Wnt2 may play an an important regulating functionvelopment in dairy cows. New molecular targets are found to solve the placenta dysplasia.