铁皮石斛对干燥综合征患者的治疗作用及其可能机制的研究

The effect of tin caulis dendrobii on the treatment of patients with Sjogren′s syndrome and its possible mechanism

目的探讨铁皮石斛对干燥综合征患者的治疗作用及其可能机制。方法选取20例病理活检诊断为干燥综合征的受试者,将其随机分为两组(服药组和未服药组),每组10例。取同期进行体检的10例健康者作为对照组。服药组连续服用铁皮石斛(每天3次,每次5mL),而未服药组和对照组用生理盐水代替。给药1周后,检测干燥综合征患者的唾液流量、唇腺病理分级及外周血中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β),以及颌下腺水通道蛋白5(AQP5)基因和蛋白的表达情况。结果服药组患者唾液流量显著高于未服药组患者和对照组,差异有统计学意义(P<0.05);服药组患者外周血中TNF-α、IL-1β表达明显低于未服药组和对照组,差异有统计学意义(P<0.05);服药组唇腺中AQP5mRNA表达则显著高于未服药组;此外,相关分析显示TNF-α、IL-1β和AQP5与唇腺的病理分级具有直线相关性,差异有统计学意义(P<0.05)。结论铁皮石斛可通过改变干燥综合征患者TNF-α、IL-1β和AQP5mRNA的表达,从而改善腺体的病理状态,因而对干燥综合征具有治疗作用。

Objective To study the therapeutic effects and possible mechanism of tin caulis densrobii in patients with Sjogren′s syndrome.Methods Twenty patients with Sjogren′s syndrome were randomly divided into two groups（treated group and non-treated group）,every group had 10 cases.And 10 healthy people were selected as control group.Treated group was given tin caulis dendrobii,three times a day,5 mL per day.Non-treated group and control group were given normal saline instead.After one week treatment,salivary flow,pathological grade of labial gland,and the expression of tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β）,aquaporin-5（AQP5）in peripheral blood were detected.Results The salivary flow in treated group was significantly higher than that of the non-treated group and control group（P〈0.05）.The expression of TNF-αand IL-1βin treated group were significantly lower than those in non-treated group and control group（P〈0.05）,while the expression of AQP5 in treated group was significantly higher when compared to the patients without treatment（P〈0.05）.Besides,correlation analysis showed that the expressions of TNF-α,IL-1βand AQP5 had a linear correlation with pathological grade of the labial gland（P〈0.05）.Conclusion Tin caulis dendrobii could improve the pathological status of the labial gland by regulating the expression of TNF-α,IL-1βand AQP-5 mRNA,and thus it has a therapeutic effect on the patients with Sjogren′s syndrome.