铅诱导蚕豆根尖细胞产生的各种染色体畸变研究

Study on Various Chromosomal Aberration from Vicia faba Root Tip Cells Induced by Pb2

[目的]研究铅对蚕豆根尖细胞诱导产生的各种染色体畸变.[方法]用不同浓度的PbCl_2溶液培养蚕豆种子,以蒸馏水培养作为对照,培养4~7 d,每天随机取根尖固定、染色、压片后镜检,观察各种染色体畸变,并统计相应的数目.[结果]培养第4~7天,1.0×10^(-4)~5.0×10^(-4)mol/L Pb^(2+)处理组的各种染色体畸变数目都高于对照组的染色体畸变数目,且Pb2+浓度越大,染色体畸变数目越多.[结论]Pb^(2+)对蚕豆根尖细胞染色体畸变的影响程度与Pb2+处理浓度以及处理时间有关.

[ Objective] To study the effects of Pb2＋ on the different chromosomal aberrations of Viciafaba root tip cells. [ Method] Vicia faba seeds were cultured with PbCl2 solutions at different concentration, and distilled water treatment as control. The root tips were fixed, dyed and squashed for microscopic examination. [ Result] When seeds were treated with 1. 0 ×10^4 - 5. 0 ×10^ 4 m o l/L Pb2＋ when culturing 4- 7 days ,the chromosomal aberrations of Viciafaba were higher than the control group. The chromosomal aberrations increased successively with in -creasing Pb2＋ concentration. [Conclusion] The influence degree of Pb2＋ on chromosomal aberrations of Viciafaba root tip cells is related to Pb2＋ concentration and treating time.