黄芪注射液联合恩替卡韦对乙型肝炎模型小鼠血清炎症因子及肝功能的影响

Effects of Astragali Radix Injection Combined with Entecavir on Serum Inflammatory Cytokines and Liver Function of Model Mice with Hepatitis B

目的:研究黄芪注射液联合恩替卡韦对乙型肝炎模型小鼠血清炎症因子及肝功能的影响。方法:将60只小鼠随机分为正常对照组(生理盐水)、模型组(生理盐水)、黄芪注射液组(0.5 mL/10 g)、恩替卡韦组(45μg/kg)和联用组(0.5 mL/10 g黄芪注射液+45μg/kg恩替卡韦),各12只。除正常对照组外,其余4组小鼠均建立乙型肝炎模型。成模后,各组小鼠ig给药,每天1次,连续4周。给药结束后,检测小鼠血清中肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)、白细胞介素8(IL-8)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、总胆红素(TBIL)水平,观察各组小鼠肝组织的病理学变化。结果:与正常对照组比较,模型组小鼠血清中TNF-α、IFN-γ、IL-8、AST、ALT和TBIL水平均显著升高(P<0.05),肝细胞呈弥散性重度脂肪变性。与模型组比较,各给药组小鼠血清中TNF-α、IFN-γ、IL-8、AST、ALT和TBIL水平均显著降低(P<0.05),肝组织病理学变化得到不同程度改善,且联用组小鼠指标改善程度优于黄芪注射液组和恩替卡韦组(P<0.05)。结论:黄芪注射液联合恩替卡韦有助于下调乙型肝炎模型小鼠血清中炎症因子表达、改善肝功能水平,且两药联用效果优于两药单用。

OBJECTIVE：To study the effects of Astragali radix injection combined with entecavir on serum inflammatory cytokines and liver function of model mice with hepatitis B. METHODS：60 mice were randomly divided into normal control group（normal saline）,model group（normal saline）,Astragali radix injection group（0.5 mL/10 g）,entecavir group（45 μg/kg）and combination group（0.5 mL/10 g Astragali radix injection＋45 μg/kg entecavir）,12 in each group. Except for normal control group,mice in other 4 groups were induced for hepatitis B models. After modeling,all mice were intragastrically administrated once aday,for 4 weeks. After administration,tumor necrosis factor α（TNF-α）,interferon γ（IFN-γ）,interleukin 8（IL-8）,aspartate aminotransferase（AST）,alanine aminotransferase（ALT）,total bilirubin（TBIL）levels in serum were detected,and the pathological changes in liver tissue in each group were observed. RESULTS：Compared with normal control group,TNF-α,IFN-γ,IL-8,AST,ALT,TBIL levels in serum of mice in model group were significantly increased（P0.05）;and liver cells were diffuse and severe steatosis. Compared with model group,TNF-α,IFN-γ,IL-8,AST,ALT,TBIL levels in serum of mice in each administration group were significantly decreased（P0.05）,pathological changes in liver tissue were improved to varying degrees,and the index improvement degree in combination group was superior to Astragali radix injection group and entecavir group（P0.05）.CONCLUSIONS：Astragali radix injection combined with entecavir helps to down-regulate the expressions of inflammatory cytokines of model mice with hepatitis B,and improve the liver function. The combination use has better effect than single use.