维药毛菊苣95%乙醇提取物对刀豆蛋白A诱导小鼠免疫性肝损伤的保护作用及机制研究

Study on the Protective Effect and Mechanism of Wei Medicine Cichorium glandulosum 95% Ethanol Extract on Con-A-induced Immunological Liver Injury in Mice

目的:考察维药毛菊苣95%乙醇提取物(CG-I)对小鼠免疫性肝损伤的保护作用及机制,为后期筛选其有效部位提供参考。方法:将60只小鼠随机分为空白对照组(生理盐水)、模型组(生理盐水)、阳性对照组(甘草酸二铵,100 mg/kg)和CG-I高、中、低剂量组(以生药量计分别为200、100、50 g/kg),每组10只,每天ig给药1次,连续给药10 d。末次给药后1 h,除空白对照组外的其余各组小鼠均通过尾iv刀豆蛋白A(Con-A)诱导免疫性肝损伤。造模8 h后,检测小鼠血清中肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)和白细胞介素1β(IL-1β)含量,计算肝、脾指数,观察肝组织病理学变化并检测肝组织中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、谷胱甘肽S-转移酶(GST)、碱性磷酸酶(AKP)、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)水平。结果:与空白对照组比较,模型组小鼠血清中TNF-α、IFN-γ、IL-1β含量显著增加,肝、脾指数及肝组织中AST、ALT、GST、AKP、T-SOD水平显著升高,肝组织中MDA水平显著降低,差异均有统计学意义(P<0.05或P<0.01);小鼠肝索排列杂乱,肝细胞出现肿胀、坏死等病变。与模型组比较,除CG-I低剂量组小鼠肝组织中AST、ALT、AKP水平以及CG-I中、低剂量组小鼠血清中MDA、IFN-γ含量降低不显著外,其余各指标均显著改善(P<0.05或P<0.01);肝组织病理变化均不同程度地减轻。结论:CG-I对Con-A所致小鼠免疫性肝损伤具有保护作用,尤其以高、中剂量效果较好;其机制可能与抗氧化、抗炎作用有关。

OBJECTIVE：To investigate the protective effect and mechanism of Wei medicine Cichorium glandulosum 95% ethanol extract（CG-I）on immunological liver injury in mice,and provide reference for post-screening its effective site. METHODS：60 mice were randomly divided into blank control group（normal saline）,model group（normal saline）,positive control group（Diammonium glycyrrhizinate,100 mg/kg）and CG-I high-dose,medium-dose,low-dose groups（calculated by crude drugs as 200,100,50 g/kg）,10 in each group,intragastrically administrated once every day,for 10 d. After 1 h of last administration,except for blank control group,mice in other groups were intravenously injected Con-A in tail to induce immunological liver injury. After 8 h of modeling,tumor necrosis factor α（TNF-α）,interferon γ（IFN-γ）,interleukin 1β（IL-1β）contents in serum were detected;liver and spleen indexes were calculated. The pathological changes in liver tissue were observed,and aspartate aminotransferase（AST）,alanine aminotransferase（ALT）,glutathione S-transferase（GST）,alkaline phosphatase（AKP）,total superoxide dismutase（T-SOD）,malondialdehyde（MDA）levels in liver tissue were detected. RESULTS：Compared with blank control group,TNF-α,IFN-γ,IL-1β contents in serum in model group were significantly increased;liver,spleen indexes and AST,ALT,GST,AKP,T-SOD levels in liver tissue were significantly increased;and MDA level in liver tissue was significantly reduced,with statistical significances（P0.05 or P0.01）;liver of mice in model group was cluttered,showing swelling,necrosis and other diseasesin liver cells. Compared with model group,except that AST,ALT,AKP levels in liver tissue in CG-I low-dose group and MDA,IFN-γ contents in serum in CG-I medium-dose,lowdose groups had no significant decrease,other indexes were significantly improved（P0.05 or P0.01）;and pathological changes in liver tissue were relieved to varying degrees.CONCLUSIONS：CG-I shows protective effect on Con-A-inducedimmunological liver injury in mice,especially the high dose and medium dose. The mechanism may be associated with its anti-oxidation and anti-inflammatory effects.