T细胞斑点试验在结核性胸膜炎诊断中的应用

Application of T-SPOT.TB in diagnosis and differential diagnosis of tuberculous pleurisy

目的观察T细胞斑点试验在结核性胸膜炎诊断中的应用价值。方法选取结核性胸膜炎患者54例为观察组,同期确诊为非结核性胸腔积液患者50例为对照组。所有患者均采用T细胞斑点试验、血清结核抗体(TB-AB)、腺苷脱氨酶(ADA)、胸腔积液结核分枝杆菌培养检测,比较各个检测方法诊断准确情况。结果观察组斑点形成细胞(SFC)形成数量均高于对照组,差异有统计学意义(P<0.05);观察组抗原检测SFC阳性率均高于对照组,差异有统计学意义(P<0.05);T细胞斑点试验诊断准确率高于其他检测方法,差异有统计学意义(P<0.05)。结论 T细胞斑点试验用于临床诊断结核性胸膜炎具有极高的敏感度、特异度,诊断准确率高。

Objective To observe the application value of T-SPOT. TB in diagnosis and differential diagnosis of tuberculous pleurisy. Methods Fifty-four patients with tuberculous pleurisy were selected as observation group,and 50 patients with non-tuberculous pleural effusion were selected as control group. All patients took T-SPOT. TB,TB-Ab,ADA,Mtb culture detection. The diagnostic accuracy of each detection method were compared. Results The SFC quantity of observation group was higher than that of control group（ P〈0. 05）; The SFC positive rate of each antigen detection of observation group was higher than that of control group（ P〈0. 05）; The diagnostic accuracy of T-SPOT. TB was higher than that of other detection methods（ P〈0. 05）. Conclusions T-SPOT. TB has higher sensitivity,specificity and high diagnostic accuracy in clinical diagnosis of tuberculous pleurisy.