间接法建立杭州地区妊娠中期孕妇血清微量元素参考区间

Establishment of reference intervals for serum trace elements of mid-term pregnant women in Hangzhou by indirect method

目的探讨并验证使用间接法建立杭州地区妊娠中期孕妇血清微量元素参考区间的可行性和可靠性。方法回顾性分析浙江省人民医院检验中心2015年1月-2016年12月3 406例妊娠中期孕妇铜、铁、锌检测结果,同时收集151例育龄期健康妇女作为对照组,使用非参数排序法获得参考区间。另选择20例健康妊娠中期孕妇验证参考区间。结果妊娠中期孕妇组Cu为(30.44±5.27)μmol/L;Fe为(15.79±6.63)μmol/L;Zn为(9.88±1.67)μmol/L,与对照组比较,Cu、Fe、Zn差异均有统计学意义(P<0.05)。使用杜奇法联合非参数排序法计算获得的孕妇微量元素参考区间,Cu:22.16μmol/L^39.53μmol/L、Fe:5.9μmol/L^24.3μmol/L、Zn:7.26μmol/L^12.18μmol/L。间接法建立的参考区间用20例健康孕妇参考个体验证通过。结论通过间接法建立妊娠中期孕妇血清微量元素参考区间,方法简单、可靠,为临床医生对本地区孕妇提供营养保健指导有重要意义。

Objective To investigate and testify the feasibility and reliability of reference intervals for serum trace elements of mid-term pregnant women in Hangzhou region by indirect method. Methods The test results of serum copper（ Cu）,iron（ Fe）,zinc（ Zn） from 3 406 mid-term pregnant women in Zhejiang Province People＇s Hospital from January 2015 to December2016 were retrospectively analyzed. 151 women at child-bearing age were chosen as the control group. Nonparametric ranking method was used to establish the reference intervals. In addition,20 healthy individuals＇ results in the mid-term pregnancy were tested to verify the reference intervals. Results The concentrations of serum trace elements in mid-term pregnant women were Cu（ 30. 44 ± 5. 27） μmol/L,Fe（ 15. 79 ± 6. 63） μmol/L,Zn（ 9. 88 ± 1. 67） μmol/L,and compared with the control group,there was statistical significance on the differences between these two groups（ P〈 0. 05）. The reference intervals of trace elements in pregnant females were calculated using the way of combination of the Tukey method with the nonparametric ranking method,and the intervals were as the follows： Cu（ 22. 16 μmol/L-39. 53 μmol/L）,Fe（ 5. 9 μmol/L-24. 3 μmol/L）,Zn（ 7. 26 μmol/L-12. 18 μmol/L）,which were verified by 20 healthy pregnant women. Conclusion Indirect method can be applied for the establishment of reference intervals in mid-term pregnant women. These reference intervals can offer a significant and meaningful guidance in a nutritional health-care for the pregnant women.