栉孔扇贝酶解液制备条件优化及其抗氧化性研究

Study on Optimization of Preparation Conditions and Antioxidant Activity of Enzymatic Hydrolysate of Chlamys farreri

以栉孔扇贝为原料,以水解度和DPPH自由基清除率为指标,在单因素试验基础上,采用Box-Behnken方法,通过响应面试验优化酶解制备具有抗氧化性多肽的扇贝酶解液工艺,并对扇贝酶解液的DPPH自由基清除率、羟自由基清除率、总抗氧化活力和抗超氧阴离子活力进行测定。研究了蛋白酶种类、酶解时间、酶解温度、加酶量和固液比对栉孔扇贝酶解液抗氧化性的影响。结果表明:扇贝最佳酶解条件为选择中性蛋白酶、酶解时间4.7h、酶解温度43℃、加酶量3.5%、固液比(m/V)1∶5。在此条件下,DPPH自由基清除率达到68.21%,羟自由基清除率为33.74%,总抗氧化活力为0.086U/mg prot,抗超氧阴离子活力为106.27U/g prot。

With Chlarnys farreri as raw material, degree of hydrolysis and DPPH radical scavenging rate are used as indicators. Based on the single factor test, the Box-Behnken method is used to optimize the enzymatic hydrolysis of scallop hydrolysate with antioxidative polypeptide by response surface test. DPPH free radical scavenging rate, hydroxyl radical scavenging rate, total antioxidant activity and anti-superoxide anion activity are measured. The effects of protease types, enzymolysis time, enzymolysis temperature, enzyme amount and solid-liquid ratio on the antioxidant activity of enzymatic hydrolysate of Chlarnys farreri are studied. The results show that the optimal parameters of enzymolysis are as follows： using neutral protease, the enzymolysis time is 4.7 h, the enzymolysis temperature is 43 ＂C, the enzyme amount is 3.5%%, the solid-liquid ratio （m/V） is 1 ＂ 5. Under the optimum conditions, the scavenging rate of DPPH free radical and hydroxyl radical is 68. 21% and 33.74% respectively, the total antioxidant activity is 0. 086 U/mg prot, the anti-superoxide anion radical activity is 106.27 U/g prot.