高产Monacolin K红曲菌种的筛选及发酵条件的优化

Screening of Monascus with High-yield Monacolin K and Optimization of Liquid-state Fermentation Conditions

为了提高Monacolin K的产量,应用30keV的N+离子注入,对紫红曲霉M2进行诱变育种。诱变剂量分别为1×10^(15),5×10^(15),1×1016,5×1016,1×1017 N+/cm2,通过构巢曲霉对峙培养、洛伐他汀抗性筛选、高效液相色谱HPLC方法筛选高产Monacolin K的优秀突变菌株,并且通过液态发酵试验优化部分培养条件。结果显示:相对原菌株M2,最佳诱变菌株11-13产Monacolin K的能力是原菌株M2的2.3倍,连续传代5次,其高产遗传形状稳定。其最佳发酵培养条件为:温度30℃,接种量10%,转速200r/min,发酵时间14天。

In order to improve the yield of Monacolin K, Monascus purpureus M2 is mutagenized by 30 keV N＋ ion implantation. The dose is 1×10^15 , 5 ×10^15 , 1×10^16 , 5 ×10^16 , 1×10^17 N^＋/cm^2 respectively Excellent mutant strains are obtained through the screening method of plate confrontation culture of Aspergillus nidulans, Monacolin K resistance and high performance liquid chromatography （HPLC）. And the fermentation conditions are optimized by liquid-state fermentation experiments. The results show that the Monacolin K yield of the best mutant 11-13 is 2.3 times higher than initial strain M2. It remains steady after 5 generations of serial cultivation. The optimum liquid-state fermentation conditions of 11-13 are as follows：the temperature is 30 ℃, the inoculum size is 10% ,the rotating speed is 200 r/re.in, the fermentation time is 14 days.