广西地区HPV感染及其基因型分析

Prevalence and genotype distribution of human papilloma virus in Guangxi

目的人乳头瘤病毒(HPV)相关疾病的发病率逐年上升。文中研究广西地区HPV感染及其基因型别分布情况及趋势。方法回顾性分析2010年5月至2017年3月于广西壮族自治区人民医院门诊、住院患者以及体检人群共15 774例。采集脱落细胞或皮损处分泌物拭子,应用基因扩增及反向斑点杂交技术对HPV基因型别进行检测。结果15 774例研究对象中检出HPV感染者6080例,HPV总感染率为38.54%。单一型别感染率和多重型别感染率分别为25.60%(4038/15 774)和12.95%(2042/15 774)。其中HPV 6(10.54%,1663/15 774)、HPV52(3.91%,616/15 774)、HPV16(2.16%,340/15 774)、HPV11(2.14%,338/15 774)和HPV58型(2.00%,316/15 774)是单一型别感染的主要型别;在2042例多重感染者中,HPV 52+53(4.26%,87/2042)和52+58型(3.23%,66/2042)是检出率最高的多重感染型别组合,其次为HPV 16+43(2.40%,49/2042)和16+52(2.30%,47/2042)。前6位高危型HPV分别为52、16、58、51、53和18型,6种HPV总感染率为26.77%(4222/15 774,95%CI=26.08~27.46);而低危型HPV分别为6、11和43型,3种HPV总感染率为27.77%(4380/15 774,95%CI=27.07~28.47)。结论 HPV6、52、16、11、58和18是广西地区HPV感染的主要基因型别,HPV 52+53、52+58型是主要的多重感染组合,其中52型是广西地区主要的高危HPV基因型别。

Objective To investigate the prevalence and genotype distribution of human papillomavirus( HPV) in the region of Guangxi. Methods We retrospectively analyzed 15774 individuals from the Outpatient and inpatient Unit as well as Physical Examination Departments of the People's Hospital of Guangxi Zhuang Autonomous Region between May 2010 and March 2017. Exfoliated c ellsor swab specimens were collected,and the genotypes of HPV were determined by Polymerase Chain Reaction( PCR) and reverse blot assay. Results The prevalence of HPV infection was 38. 54%( 6080/15774). The infection rate of single genotype and multiple genotypes were 25.60%( 4038/15774) and 12.95%( 2042/15774),respectively. In single infection patterns,the most common genotypes included HPV 6( 10. 54%, 1663/15774),52( 3. 91%, 616/15774),16( 2. 16%,340/15774),11( 2. 14%,338/15774),and 58( 2.00%,316/15774). While among the multiple infections patterns,HPV 52+ 53( 4. 26%,87/2042) and 52 + 58( 3. 23%,66/2042) were common,and followed by HPV 16 + 43( 2. 40%,49/2042),16+52( 2.30%,47/2042),6+42( 2.15%,44/2042) and 6+43( 2.15%,45/2042).HPV 52,16,58,51,53 and 18 were the top six high risk( HR) genotypes of HPV,accounting for 26.77%( 4222/15774,95%CI = 26.08-27.46); while HPV 6,11 and43 were the leading low-risk( LR) genotypes of HPV,accounting for 27.77%( 4380/15774,95%CI = 27.07-28.47).The overall ratio of single infection to multiple infections was 1.98( 4038 vs. 2042).Conclusion HPV 6,52,16,11,58,18 were the main HPV infection genotypes,and 52+53 and 52+58 were common infection combinations in Guangxi Zhuang Autonomous region. The HPV multiple infections were increased. Apparently,more HPV low risk genotypes were seen in male patients that should be aware.