Luminex技术筛选胃癌复发外周血微小RNA指纹图谱

Screening of microRNAs fingerprints of peripheral blood in recurrent gastric cancer patients by Luminex technique

目的应用Luminex技术筛选胃癌复发外周血微小RNA（miRNA，miR）指纹图谱。方法选取胃癌复发和未复发患者各100例及对照健康人100例，应用Luminex技术筛选外周血中5种miRNAs表达，另外选择50例胃癌患者动态观察血清miRNAs表达变化，筛选胃癌复发外周血microRNAs分子组合。结果miR-10b、miR-21、miR-183、miR-371-5p、miR-378 5种miRNAs在胃癌患者术前血清中的表达显著高于健康对照组，其在胃癌组和健康对照组表达中位数分别为：miR-10b（8.25比3.25）、miR-21（11.18比3.25）、miR-183（22.86比4.13）、miR-371-5p（13.57比3.37）、miR-378（18.19比3.99），均P＝0.000。术后血清miR-10b在早期胃癌复发组与未复发组比较上调不明显（7.41比7.33，P＝0.336）。50例动态观察的患者中，复发组术后血清miR-21、miR-371-5p、miR-378、miR-183均显著下调，复发后，血清miR-21、miR-371-5p、miR-378显著上调，但miR-183上调不显著（复发组比未复发组中位数分别为1.99、1.14），提示miR-183可以从组合中剔除。结论筛选出miR-21、miR-371-5p、miR-378 3种miRNAs分子组合作为胃癌复发外周血microRNAs指纹图谱，能够早期预测胃癌复发。

ObjectiveTo screen the microRNAs （miRNA, miR） fingerprint of peripheral blood in patients with recurrent gastric cancer by Luminex technique.Methods100 patients with gastric cancer recurrence, 100 patients with non-recurrence and 100 heathy controls were recruited in this study. Five kinds of miRNAs in peripheral blood were screened by Luminex technique. In addition, 50 patients with gastric cancer were collected and miRNAs expression in serum was detected dynamicly to select microRNAs fingerprint in serum of patients with recurrent gastric cancer.ResultsThe expression of five kinds of miRNAs in preoperative serum of patients with gastric cancer was significantly higher than in the healthy controls [The median expression in gastric cancer and healthy controls was as follow： for miR-10b （8.25 vs. 3.25）; for miR-21 （11.18 vs. 3.25）; for miR-183 （22.86 vs. 4.13）; for miR-371-5p （13.57 vs. 3.37）; and for miR-378 （18.19 vs. 3.99）, all P=0.000）. Postoperative serum miR-10b in patients with early gastric cancer recurrence was not significantly upregulated as compared with that in the patients with early gastric cancer non-recurrence （median： 7.41 vs. 7.33, P=0.336）. The expression of miR-21, miR-371-5p, miR-378 and miR-183 in 50 patients with gastric cancer was significantly downregulated after operation and significantly upregulated when recurrence. The miR-183 was removed from fingerprint due to miR-183 was not significantly upregulated when recurrence （The median： 1.99 vs. 1.14）.ConclusionThe three miRNAs （miR-21, miR-371-5p and miR-378） were screened as the specific miRNAs diagnostic fingerprint of gastric cancer recurrence and could predict early recurrence of gastric cancer.