‘金都一号’火龙果组织培养之外植体处理优化及不定芽诱导

Explant Process Optimization and Induction of Adventitious Bud of Pitaya ‘Jindu No.1'

以红皮红肉型火龙果品种‘金都一号’茎段为材料,开展火龙果外植体选择方式、外植体最佳消毒处理方式、不定芽诱导培养等组织培养试验。结果表明:火龙果外植体最好选择无病害健壮的近成熟茎;火龙果外植体最佳处理方式为不去刺座,75%酒精快速消毒30 s,0.1%升汞消毒10 min,最后无菌水冲洗5~8次;火龙果的最佳不定芽诱导培养基为MS+4.0 mg/L 6-BA+0.1 mg/L NAA,诱导率为80%。

This study used red-flesh dragon fruit variety-＂Jindu No.1＂ stem as the material, to research the best way of explant selection, explant disinfection treatment, adventitious bud induction in the pitaya tissue culture experiment. The results showed that pitaya explant had better choose healthy and robust nearly mature stem; and the most suitable disinfection treatment for pitaya stem segments was keeping thorn and followed by rinsing in 75%alcohol for 30 s, then rinsed 0.1% mercuric chloride disinfection for 10 min, and finally rinsed with sterile water5-8 times. The best culture medium for adventitious bud induction of pitaya was MS ＋4.0 mg/L 6-BA ＋0.1 mg/L NAA, and induction rate was 80%.