米糠提取物对PC12细胞保护作用的体外实验研究

AN EXPERIMENTAL STUDY ON THE PROTECTIVE EFFECTS OF RICE BRAN EXTRACTS ON PC12 CELLS IN VITRO IN VITRO

目的探讨富含γ-氨基丁酸(γ-aminobutyric acid,GABA)的米糠提取物(rice bran extracts,RBE)对Aβ_(25-35)致PC12细胞损伤的影响。方法 (1)将细胞分为对照组和不同浓度RBE处理组,RBE的浓度分别为50、100、200、400、800、1600和3200(μg/ml),用CCK-8法检测RBE对PC12细胞活性的影响。(2)将细胞分为对照组(不加任何处理因素)、Aβ_(25-35)处理组(20μmol/L)、不同浓度RBE干预+Aβ_(25-35)处理组,RBE浓度分别为100、200、400、500、800、1000和1600(μg/ml)。RBE干预采用预孵育和共孵育2种方式,用CCK-8法检测RBE对PC12细胞的保护作用;并比较2种不同孵育方式的保护效果。结果 (1)一定浓度范围内的RBE可提高PC12细胞的活性。(2)RBE预孵育的浓度为200~1000μg/ml时,对Aβ_(25-35)损伤的PC12细胞具有显著保护作用(P<0.05),其最佳浓度为800μg/ml;RBE共孵育的浓度为400~1000μg/ml时,对Aβ_(25-35)损伤的PC12细胞具有显著保护作用(P<0.01),其最佳浓度为1000μg/ml。结论适宜浓度的米糠提取物干预对Aβ_(25-35)损伤的PC12细胞具有保护作用。

Objective To investigate the neuroprotective effects of rice bran extracts （RBE） rich in 7-aminobutyric acid （GABA） on PC12 cells. Methods 1. PC12 cells were divided into control group and RBE treated groups （at the concentrations of 50, 100, 200, 400, 800, 1600, 3200 pg/ml, respectively）. Cell viability was determined by CCK-8 assay. 2. PC12 cells were divided into control group, A[~25.35 treated group and RBE＋A[325.35 treated groups （RBE at the concentrations of 100, 200, 400, 500, 800, 1600 gg/ml, respectively）. The cells was pretreated with RBE before exposed to A925-35 or co-treated with RBE and A[~25.35. Cell viability was determined by CCK-8 assay. Results （1） Suitable concentrations of RBE increased cell viability of PC12 cells. （2） RBE pre-treatment at the concentrations of 200-1000 mg/ml had significant protective effects on PC12 cells exposed to A325.35 （P〈0.05）, and 800 mg/ml was considered the most suitable concentration. RBE co-incubation at the concentrations of 400-1000 mg/ml had significant protective effects on PC12 cells exposed to A1325-35 （P〈0.01）, and 1000 mg/ml was considered the most suitable concentration. Conclusion Suitable concentrations of rice bran extracts have protective effects against injury induced by A^25.35 in PC12 cells.