摘要
目的建立桦褐孔菌氨基酸含量测定方法。方法样品用含0.1%巯基乙酸的盐酸溶液水解提取,样液经定容、过滤、浓缩、复溶、过膜后,用氨基酸分析仪测定,外标法定量。结果应用含巯基乙酸的盐酸水解液,对样品进行水解的同时有效防止了蛋氨酸氧化,方法回收率为93%~97%,相对标准偏差小于2.6%。结论该方法准确快速、重现性好,适用于桦褐孔菌氨基酸含量测定的要求,可为今后相关产品的质量评价提供参考。
Objective In this study, a new method for determination of 17 amino acids in Inonotus obliquus was established using an amino acid analyzer （AAA）. Methods The samples were hydrolyzed in hydrochloric acid solution containing 0.1% thioglycolic acid, and the contents of amino acids were determined by AAA. The external standard method was used for quantitative analysis. Results This hydrolysis pretreatment was effectives in preventing oxidation of methionine. The recovery rates were 93%-97%, and the relative standard deviations were less than 2.6 %. Conclusion The method was accurate, and of good repeatability, which could meet the requirements for determination of 17 amino acids in Inonotus obliquus, and provides reference for the quality evaluation of related products.
出处
《营养学报》
CAS
CSCD
北大核心
2017年第5期504-506,共3页
Acta Nutrimenta Sinica
关键词
氨基酸
桦褐孔菌
氨基酸分析仪
amino acids
lnonotus obliquus
amino acid analyzer
