鸡Ⅱ型抗菌肽LEAP-2的原核表达及抑菌研究

Study on Prokaryotic Expression and Bacteriostasis Activity of Chicken Type Ⅱ Antimicrobial Peptide LEAP-2

为了研究鸡Ⅱ型抗菌肽LEAP-2的体外表达产物的活性,试验进行了肝脏样品无菌采集,基因的克隆及密码子优化和表达,以表达产物对致病菌做纸片法药敏试验,判断其抑菌作用。结果表明,试验经低温处理获得了较好的肝脏总RNA,克隆并优化了鸡LEAP-2基因;经诱导得到了表达产物,形式为包涵体;抑菌试验表明,表达产物对16株分离致病菌具有抑制作用。

To determine the antibiotic activity of chicken LEAP-2 expressed in vitro, the gene of LEAP-2 was applied by RT-PCRinstructed by the sequence NM_001001606, and then was done with codon optimization and cloned into pGEX-6P-1. After expressed inE. coli DE3, the expressed product was managed to do antibiotic experiment by bacteriostasis circle test. The results showed that chickenLEAP-2 gene hada satisfied gain under liquid nitrogen processing, LEAP-2 was cloned and after optimized then expressed by incusionbodies type, antimicrobial susceptibility test result showed that 16 E. coli strain owned in laboratory was sensitived to the protein.