黄芪和泼尼松对阿霉素肾损伤的保护作用

Prednisone and the Protective Effect of Fangjihuangqi Decoction on Adriamycin-induced Nephropathy

目的观察防己黄芪汤和泼尼松对阿霉素肾损伤的保护作用,初步探讨其作用机制。方法从兔耳缘静脉手推阿霉素2.0 mg/kg·次×1次/周×4周造模,注射后第5周,测24 h尿蛋白超过100 mg为阿霉素肾病模型建成。将模型兔随机均分为4组,分别给予常规饲料(模型组)、防己黄芪汤(黄芪组)、泼尼松(泼尼松组)、防己黄芪汤+泼尼松(联合用药组)。8周后,检测血生化,观察肾组织病理改变。结果模型组肾小球呈弥漫性硬化,说明动物建模成功;黄芪组、泼尼松组和联合用药组肾小球均有不同程度的硬化,病变轻于模型组。Scr、BUN值,模型组高于药物干预组,差异有统计学意义(P<0.05)。结论泼尼松和黄芪都具有降低阿霉素所致肾损伤的作用,其药效与联合用药近似。

Objective To explore the effect of Fangjihuangqi decoction and prednisone on adriamycin-induced nephropathy, and investigate its mechanisms. Methods All rabbits were treated by adriamycin injection via ear vein（2.0 mg/kg, once a week, lasting 4 weeks）. After 5 weeks, 24 h urine protein was tested and24 h UP over 100 mg was regarded as mark of adriamycin-induced nephropathy being built successfully. Model rabbits were randomly divided into 4 groups and treated with normal diet（model control group）, Fangjihuangqi decoction（Huangqi group）, prednisone（prednisone group） and combination of Fangjihuangqi decoction and prednisone（combination group）, respectively. Blood biochemical tests as well as pathologic evaluation were carried out at the end of 8 th week. Results Diffuse glomerular sclerosis presented in all model controls, which demonstrated success in modeling. All the other groups also presented glomerular sclerosis but less severe. Scr and BUN were significantly higher in intervention groups than in model control（P0.05）. Conclusions Fangjihuangqi decoction and prednisone both have protective effect on adriamycin-induced nephropathy.