UCP2 45bp-I/D基因多态性与代谢综合征的关系

Relationship between UCP2 45bp-I/D gene polymorphisms and metabolic syndrome in Han Chinese population

目的探讨解偶联蛋白2(UCP2)45bp-I/D基因多态性与代谢综合征(Met S)的关系。方法选取杭州市采荷小区40岁以上汉族人群作为研究对象,符合2005年国际糖尿病联盟(IDF)Me t S诊断标准者纳入Me t S-IDF组,共95例;符合2013年中华医学会糖尿病学分会(CDS)Met S诊断标准者纳入Met S-CDS组,共78例;代谢指标完全正常者为正常组(N组),共109例,并进行UCP2基因多态性分型,分析不同诊断标准Met S组与N组间基因型的区别,并行Binary logistic回归分析不同基因型中Met S各相关组分的区别。结果 UCP2基因频率在Met S-IDF组为D/D 72.63%、D/I+I/I 27.37%,Met S-CDS组为D/D 78.21%、D/I+I/I21.79%,N组为D/D 88.99%、D/I+I/I 11.01%;等位基因I频率在Me t S-IDF组为16.32%、Me t S-CDS组为13.46%、N组为7.8%。不同诊断标准的两组Met S患者基因型分布和等位基因频率与N组对比差异均有统计学意义(均P<0.05)。Binary logistic回归分析表明,Met S-IDF组中,基因型为D/I+I/I者相对于D/D者发生高甘油三酯血症、胰岛素抵抗、腰围增粗等风险增高,Met S-CDS组中,基因型为D/I+I/I者相对于D/D者发生高甘油三酯血症、胰岛素抵抗、腰围增粗、BMI升高风险增加。结论两个Met S诊断标准均显示,Met S患者与代谢指标正常者的UCP2 45bp-I/D基因型分布和等位基因频率均有显著差异,I等位基因可能是Met S的危险因素。

Objective To investigate the relationship between polymorphisms of uncoupling protein 2（UCP2） gene and metabolic syndrome（Met S） in Han Chinese population. Methods Total 760 Han Chinese residents from one community in Hangzhou were randomly selected in the study. Demographic data were collected, and metabolic index were detected. Among 760 individuals, 95 met the Met S criteria of 2005 International Diabetes Federation（IDF）（Met S-IDF group） and 78 met the Met S criteria of 2013 Chinese Diabetes Society（CDS）（Met S-CDS group）, 109 subjects with normal blood tests and physical examination served as control group. The genotypes of the UCP2 45 bp-I/D were determined with PCR-RFLP. The influencing factors of Met S were analyzed by logistic Binary regression. Results The gene frequency of D/D in Met S-IDF group and control group was 72.63%and 88.99%（P0.05）, that of D/I＋I/I was 27.37% and 11.01%（P0.05）, respectively; the frequency of allele I in Met S group and control group was 16.32% and 7.8%（P0.05）respectively. The gene frequency of D/D in Met S-CDS group and control group was78.21% and 88.99%（P0.05）, respectively, that of D/I＋I/I was 21.79% and 11.01%（P0.05）, respectively, the frequency of allele I in Met S group and control group was 13.46% and 7.8%（P0.05）, respectively. Binary logistic analysis showed that the subjects with D/I＋I/I genotype had higher risks of hypertriglyceridemia, insulin resistance and increased waist circumference than those with D/D genotype in Met S-IDF group. While the subjects with D/I＋I/I genotype had higher risks of the hypertriglyceridemia, insulin resistance, increased waist circumference and body mass index（BMI）than those with D/D genotype in Met S-CDS group.Conclusion Our study shows that the allele I of UCP2 gene might be a risk factor for metabolic syndrome in Han Chinese population.