雷帕霉素对睾丸卵黄囊瘤细胞生长及凋亡的影响

Effect of rapamycin on growth and apoptosis of testicular yolk sac tumor cells

目的研究雷帕霉素对睾丸卵黄囊瘤细胞的生长抑制和诱导凋亡作用。方法用不同浓度的雷帕霉素(1、10、50、100nmol/L)处理睾丸卵黄囊瘤细胞,设空白组和阴性对照组。采用CCK-8法测定不同浓度雷帕霉素对细胞生长的影响;采用流式细胞仪检测不同浓度雷帕霉素对细胞周期及凋亡的影响。结果雷帕霉素可以抑制睾丸卵黄囊瘤细胞生长,呈浓度依赖性和时间依赖性,除1nmol/L浓度组外,其余浓度组各时间点的细胞生长抑制率与阴性对照组比较差异均有统计学意义(均P<0.05)。雷帕霉素可以诱导睾丸卵黄囊瘤细胞凋亡,使细胞停留在G0/G1期,除1nmol/L浓度组外,其余浓度组G0/G1期细胞比例及细胞凋亡率显著高于阴性对照组,差异均有统计学意义(均P<0.05)。结论雷帕霉素能够抑制睾丸卵黄囊瘤细胞的生长,促进细胞凋亡。

Objective To investigate the effect of rapamycin on the growth and apoptosis in testicular yolk sac tumor（YST） cells.Methods The testicular YST cells were treated with rapamycin of different concentrations（1 nmol/L, 10 nmol/L, 50 nmol/L and 100 nmol/L）.The growth rate for testicular YST cells was evaluated by CCK-8 method, the cell cycle and apoptosis rate were determined by flow cytometry.Results Rapamycin inhibited the growth of testicular YST cells in a concentration and time-dependent manner（P 0.05）. Rapamycin at all concentrations except 1 nmol/L induced the apoptosis of testicular YST cells, and also induced cell cycle arrest at G0/G1 phase（P 0.05）.Conclusion Rapamycin can significantly inhibit the cell growth and induce the apoptosis of testicular YST cells.