乙肝肝硬化对外周血T细胞亚群、免疫球蛋白及补体水平的影响

Influence of hepatitis B cirrhosis on peripheral blood T lymphocyte subsets,immunoglobulin and complement levels

目的:检测乙型肝炎肝硬化患者外周血中T淋巴细胞亚群、免疫球蛋白及补体水平的变化并探讨其临床意义。方法:选取67例乙肝肝硬化患者和20例健康对照者,采用流式细胞术检测其外周血中T淋巴细胞亚群(CD4+、CD8+),采用免疫比浊法测定其血清中免疫球蛋白(IgG、IgM、IgA)及补体(C3、C4)水平。结果:乙肝肝硬化患者外周血中CD4+T、CD8+T淋巴细胞计数低于对照组(P<0.05),免疫球蛋白IgG、IgM、IgA均高于对照组(P<0.05),而补体C3、C4低于对照组(P<0.05)。结论:乙肝病毒可以影响T细胞亚群和免疫球蛋白水平,从而导致机体细胞和体液免疫功能紊乱,免疫功能的检测对评估乙肝肝硬化患者病情及判断预后有一定临床意义。

Objective :To study the changes of T-lymphocyte subsets,serum immunoglobulin and complement in patients with chronic hepatitis B(CHB)-related cirrhosis.Methods:The various T-lymphocyte subsets were detected by flow cytometry,serum immunoglobulin and complement by immune nephelometry in 67 patients with CHB-related cirrhosis and 20 casesof healthycontrols.Results:Compared to the healthycontrol group,the CHB-related cirrhosis groups showed significantly less peripheral blood T cells percentages of CD4+(P<0.05)and CD8+(P<0.05).The levels of serum IgG、IgM and IgAin the patientswith CHB-related cirrhosis were significantly higher than those in healthy group(P<0.05).The levels of C3 and C4 in the patients with CHB-related cirrhosis were significantly higher than those in healthy group(P<0.05).Conclution:The immune function was disturbed in the patients with CHB-related cirrhosis.The detection of immune function has clinical significance in the diagnosis,treatment and predict prognosis of CHB-related cirrhosis.