柴芪汤对代谢综合征大鼠肠道损伤的干预作用研究

Study on Chaiqi decoction on intestinal injury in rats with metabolic syndrome

[目的]观察柴芪汤对饮食诱发的代谢综合征(MS)模型大鼠肠道损伤的干预效果。[方法]将40只SPF级雄性SD大鼠随机分成正常组、模型组、中药组、西药组,除正常组外其余各组用高盐高脂高糖饲料喂养。中、西药组分别在造模同时给予柴芪汤、盐酸吡格列酮混悬液灌胃,正常组和模型组给予生理盐水灌胃做对照,均持续喂养16周。第16周末大鼠取材,检测血清血糖、血脂、胰岛素,评价胰岛素抵抗(IR),测量大鼠体质量、血压,测量并计算小肠推进率,苏木精-伊红(HE)染色观察肠道病理改变,以评价大鼠肠道损伤程度。Western blot法检测小肠NF-κB蛋白表达。[结果]柴芪汤组血糖、胰岛素、胰岛素抵抗指数(IRI)、血脂、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、血压水平均明显低于模型组,高于正常组(P<0.05),柴芪汤组小肠NF-κB蛋白表达较模型组明显减弱,强于正常组(P<0.05)。柴芪汤可提高小肠推进率,改善肠道病理损害。[结论]柴芪汤可改善MS大鼠的糖脂代谢紊乱,减轻MS性肠道损害的程度,延缓其进展。

[Objective] To observe the effects of Chaiqi decoction on intestinal injury of metabolic syndrome rats mode. [Methods] The forty SDF male SD rats were randomly divided into normal group, model group, traditional Chinese medicine group, western medicine group. The rat model was established by feeding high fat, high sugar and high salt diet. The Chinese medicine, Western medicine group were respectively given Chaiqi decoction, pioglitazone hydrochloride suspension from the beginning of modeling. Normal and model groups were given Physiological saline for 16 weeks. The blood glucose, blood lipids and insulin were detected at 16th week, evaluation the insulin resistance. At the same time, measure blood pressure in rats. Measure and calculate the small intestine propulsion rate. HE staining was used to observe the pathological changes of intestinal tract, to assess the degree of intestinal injury. The expression of NF-κB protein in small intestine was detected by Western blot. [Results] The blood glucose, blood lipids, insulin, insulin resistance index, IL-6,TNF-α, blood pressure in Chinese medicine Chaiqi decoction group were significantly lower than the model group, higher than the normal group(P<0.05). Chaiqi decoction can improve the rate of small intestine, improve intestinal pathological damage. [Conclusion] Chaiqi decoction can improve metabolic syndrome in rats with glucose and lipid metabolism disorders, reduce metabolic syndrome, the extent of intestinal damage, delay its progress.