四种紫堇属藏药化学成分预实验及其生物碱含量测定

Preliminary Tests for Chemical Components of Four Kinds Tibetan Medicine Herbs and Determination of Total Alkaloids

目的:采用系统预试法对斑花黄堇、迭裂黄堇、塞北紫堇和矮紫堇所含化学成分进行定性检验,并对其总生物碱含量进行测定.方法:采用浸提、索氏和超声提取法,以石油醚、95%乙醇和纯水为提取剂分别制备不同极性提取液;采用试管法、纸反应法及薄层层析法分别对提取液进行研究,通过试验指示剂和显色剂颜色或沉淀反应,检查所含化学成分;采用分光光度法测定样品中总生物碱含量.结果:斑花黄堇、迭裂黄堇、塞北紫堇和矮紫堇总生物碱含量分别是0.53、0.67、1.10和2.03 mg/g,含有氨基酸、蛋白质、多糖及苷、酚类、鞣质、生物碱、黄酮、有机酸、甾体和挥发油等化学成分.结论:明确了4种紫堇属藏药含有的化学成分类别及其总生物碱含量,为进一步研究提供实验基础.

objective：To identify the chemical components of Corydalis conspersa Maxim.,Corydalis dasyptera Maxim.,Corydalis impatiens（Pall.）Fisch.,and Corydalis hendersonii Hemsl.（C.Nemesis Kitamura）by system preliminary test,and to determine the total alkaloid content in above Tibetan medicine.Methods：The mixture was extracted with petroleum ether,ethanol and water,the chemical components were preliminarily tested using color reaction and precipitation reaction.Results：Based on the assay of chemical components,four kinds Tibetan medicine herbs contained amino acid,proteins,polysaccharides and glycosides,alkaloids,flavonoids,organic acids,etc.The contents of total alkaloids were 0.53,0.67,1.10 and 2.03 mg/g,respectively.Conclusion：These Tibetan medicine herbs consisted of many chemical components.