青光眼睫状体炎综合征患者5种常见病原微生物相关血清抗体的测定及分析

Detection and analysis on serum antibodies for five common pathogenic microbes in patients with Posner-Schlossmarm syndrome

背景青光眼睫状体炎综合征（PSS）常反复发作，最终可致盲，目前病因尚未完全阐明。研究认为一些病原微生物感染引起的免疫炎症反应与PSS的发病有一定的关联性，但大样本的临床研究鲜见报道。目的探讨PSS患者血清中巨细胞病毒（CMV）、单纯疱疹病毒（HSV）、风疹病毒（RV）、幽门螺旋杆菌（HP）及抗链球菌溶血素0（ASO）相关抗体水平，为PSS发病的影响因素及其防治研究提供依据。方法采用前瞻性病例对照研究方法，于2014年12月至2016年12月收集在深圳市眼科医院确诊的82例PSS患者的外周血各4ml，同期采集100名性别和年龄匹配的健康献血志愿者外周血作为对照。采用间接ELISA法测定各组受检者血清中CMV、HSV、RV和HP的特异性IgG及IgM阳性率；采用免疫散射比浊法检测各组受检者血清中ASO抗体的阳性率。结果PSS组受检者血清CMV-IgG、CMV．IgM、HP-IgG、HP-IgM和ASO抗体阳性率分别为22．0％、17．1％、22．0％、17．1％和17．1％，均明显高于正常对照组的5．O％、0．O％、10．0％、2．0％和7．O％，差异均有统计学意义（X^2=11．726、18．496、4．943、12．766、4．479，均P〈0．05）。PSS组受检者血清HSV-IgG、HSV-IgM、RV·IgG和RV-IgM的阳性率与正常对照组比较差异均无统计学意义（x^2=3．305、0．986、0．898、0．503，均P〉0．05）。结论CMV、HP和溶血性链球菌感染在PSS的发生和发展中可能起到一定的作用。

Background Posner-Schlossman syndrome （PSS） is often recurrent and is a cause of blindness. The etiology of PSS remains to be elucidated. It is reported that there is a certain association between pathogenic microorganisms and PSS in rather small samples. Objective This study was to analyze the related serum antibody levels of eytomegalovirus （ CMV ） , herpes simplex virus （ HSV ） , rubella virus （RV） , helicobacter pylori （HP） and anti-streptolysin O （ASO） and provide a clue for the study on pathogenesis and therapy of PSS. Methods A prospective cases-controlled study was carried out in Shenzhen Eye Hospital from December,2014 to December,2016 under the approval of Ethic Committee of this hospital and informed consent of each subject prior to initial of any medical examination. Peripheral blood samples were collected from 82 PSS patients as the PSS group and 100 age-and gender-matched healthy blood donors as the normal control group. The positive rates of serum CMV IgG, CMV IgM,HSV IgG, HSV IgM, RV IgG, RV IgM, HP IgG and HP IgM in the subjects were detected by indirect ELISA,and the positive rate of serum ASO antibody was determined by immuno-scatter turbidmetry. Results The positive rates of serum CMV-IgG, CMV-IgM, HP-IgG, HP-IgM and ASO antibody were 22.0% , 17.1% , 22.0% , 17.1% and 17.1% in the PSS group,which were significantly higher than 5.0% ,0. 0% ,10.0% ,2.0% and 7.0% in the normal control group （X2：11. 726,18. 496,4. 943,12. 766,4. 479 ,all at P〈0.05 ）. The positive rates of serum HSV-IgG,HSV-IgM,RV-IgG and RV-IgM in the PSS group were not significantly different fi＇om those in the normal control group （X^2 = 3.305,0.986,0.898,0.503, all at P〉0.05）. Conclusions CMV,HP and hemolytic streptococcal infection may participate in the occurrence and development of PSS.