RNA干扰技术干扰信号通路在后发性白内障防治中的应用被引量：3

Application of RNA interference in posterior capsule opacification by interfering signaling pathway

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摘要后发性白内障即后囊膜混浊（PCO），是白内障超声乳化摘出联合人工晶状体植入术后常见的并发症。白内障术后残留的晶状体上皮细胞（LECs）向后囊膜表面移行、增生及上皮一间质转化（EMT）等细胞生物学行为的改变可引起LECs纤维化、PCO及囊袋皱缩。转化生长因子p2（TGF．132）是目前已知的参与诱导LECs的EMT及组织病理性纤维化关键的细胞因子，它可通过Smad经典通路参与诱导LECs的EMT过程。除此之外，P13K／AKT／mTOR信号通路也参与了TGF-β2诱导的EMT过程。RNA干扰（RNAi）技术作为基因调控手段之一，在通过干扰信号通路而抑制LECs的纤维化及增生、迁移等生物学行为中有一定的应用前景。本文就RNAi技术通过干扰P13K／AKT／mTOR信号通路和TGF-β2／Smad信号通路对LECs的生物学行为的影响，及其在PCO防治中的作用进行综述。 Posterior capsule opacification （ PCO ） is common complication after cataract phaeoemulsification with intraocular lens （IOL） implantation. The mechanism of lens epithelial ceils （LECs） fibrosis,PCO and capsular wrinkle is mainly related with residual LECs migration, proliferation and epithelial- mesenchymal transition （EMT） after cataract surgery. Transforming growth factor β2 （TGF-β2） has been proposed as the most important factor driving the EMT and pathologic fibrosis of LECs,TGF-β2 induces LECs EMT by the Smad signaling pathway. Besides,PI3K/AKT/mTOR signaling pathway was studied to participate in TGF-β2 induced EMT. As one of the gene regulation methods, RNA interference （RNAi） technology shows an important application prospect in inhibiting LECs fibrosis and proliferation by interfering signaling pathway. This review highlights RNAi effects on LECs biological behavior by interfering PI3K/AKT/mTOR and TGF-β2/Smad signaling pathways.

作者席亚慧刘红玲

机构地区哈尔滨医科大学附属第一医院眼科

出处《中华实验眼科杂志》 CAS CSCD 北大核心 2017年第12期1126-1129,共4页 Chinese Journal Of Experimental Ophthalmology

基金国家自然科学基金项目（81301325）

关键词晶状体上皮细胞后发性白内障 RNA干扰转化生长因子Β2 Lens epithelial cells Posterior capsule opacification RNA interference Transforming growthfactor β2

分类号 R776.1 [医药卫生—眼科]

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参考文献2

1秦贤杰,彭燕一.RNA干扰技术与眼科疾病[J].国际眼科杂志,2014,14(5):849-851. 被引量：2
2王逸涵,柳林.慢病毒载体介导雷帕霉素靶蛋白沉默对体外人晶状体上皮细胞增生的抑制作用[J].中华实验眼科杂志,2015,33(4):328-332. 被引量：3

二级参考文献26

1Gotoh N, Perdue NR, Matsushima H, et al. An in vitro model of posterior capsular opacity: SPARC and TGF-β2 minimize epithelial-to- mesenchymal transition in lens epithelium [J]. Invest Ophthalmol Vis Sci ,2007,48 (10) : 4679-4687.
2Yazici AT,Bozkurt E,Kara N, et al, Long-term resuhs of phacoemulsification combined with primary posterior curvilinear capsulorhexis in adults[ J ]. Middle East Afr J Ophthalmol,2012,19 ( 1 ) : 115 - 119.
3Varga A, Sacu S, V6csei-Marlovits PV, et al. Effect of posterior capsule opacifieationon macular sensitivity [ J ]. J Cataract Refract Surg,2008, 34 ( 1 ) : 52-56.
4Sarbassov DD, All SM, Sabatini DM. Growing roles for the roTOR pathway[J]. Curr Opin Cell Biol,2005,17 (6) : 596-603.
5Jewell JL, Russell RC, Guan KL. Amino acid signalling upstream of roTOR[ J]. Nat Rev Mol Cell Biol,2013,14 ( 3 ) : 133 - 139.
6Saqcena M,Menon D, Patel D, et al. Amino acids and roTOR mediate distinct metabolic checkpoints in mammalian G1 cell cycle [ J/OL ]. PloS One ,2013,8 ( 8 ) : e74157 [ 2014-04-24]. http ://journals. plos. org/plosone/artiele? id = 10. 1371/journal. pone. 0074157.
7Laplante M, Sabatini DM. roTOR signaling at a glance [ J ]. J Cell Sci, 2009,122 (20) : 3589-3594.
8Sakuma Barry MA, Ikeda Y. Lentiviral vectors : basic to translational [ J ]. Biochem J, 2012,443 ( 3 ) : 603 -618.
9Rozen-Zvi B, Hayashida T, Hubchak SC, et al. TGF-β/Smad3 activates mammalian target of rapamycin complex-1 and promotes collagen production by increasing HIF-1α expression [ J]. Am J Physiol Renal Physiol, 2013 305 ( 4 ) : F485 -494.
10Gulhati P, Bowen KA, Liu J, et al. mTORC1 and mTORC2 regulate EMT,motility,and metastasis of colorectal cancer via RhoA and Racl signaling pathways[ J ]. Cancer Res, 2011,71 ( 9 ) : 3246 - 3256.