Prdx2基因表达情况对结直肠癌SW480细胞影响作用分析

Analysis of the effect of Prdx2 gene expression on SW480 cells in colorectal cancer

目的探讨硫氧还蛋白过氧化物酶2(Prdx2)基因表达对结直肠癌SW480细胞增殖、侵袭及凋亡的影响。方法采用慢病毒空载体(空白组)、干扰片段(Prdx2-shRNA)慢病毒载体转染结直肠癌SW480细胞(实验组),野生型结直肠癌SW480细胞作为对照组,分别于转染后培养24 h、48 h、72 h、96 h、120 h采用MTT法检测SW480细胞增殖情况,采用流式细胞仪技术检测培养120 h后细胞的凋亡率,采用实时荧光定量-聚合酶链反应(qRt-PCR)及Westernblot法检测Prdx2、Survivin mRNA及蛋白的表达,采用Transwell侵袭实验检测SW480细胞的侵袭能力。结果实验组的W480细胞凋亡率显著高于空白组和对照组(P<0.05),培养48 h、72 h、96 h、120 h,实验组的W480细胞增殖能力明显低于空白组和对照组(P<0.05),实验组的SPrdx2、Survivin mRNA及蛋白相对表达水平显著低于空白组和对照组(P<0.05);Transwell侵袭实验结果显示,实验组的转染结直肠癌SW480细胞侵袭能力强于空白组和对照组(P<0.05)。结论 Prdx2基因沉默会抑制结直肠癌SW480细胞的增殖、促进凋亡,但是会增强结直肠癌SW480细胞的侵袭能力。

Objective To investigate the effect of the expression of Prdx2 gene on the proliferation,invasion and apoptosis of human colorectal cancer SW480 cells. Methods The lentiviral vector（ blank group）,interference fragment（ Prdx2-shRNA） lentiviral vector transfection of colorectal cancer cells SW480（ experimental group）,wild type colorectal cancer SW480 cells as the control group. After transfection respectively 24 h,48 h,72 h,96 h,120 h,MTT method was used to detect the proliferation of SW480 cells,the rate of cell apoptosis after 120 h was detected by culture flow cytometry. Prdx2,Survivin protein and mRNA were detected by real-time quantitative polymerase chain reaction（ qRt-PCR） expression and Western-blot method. The SW480 cell invasion ability of invasion were detected by Transwell. Results W480 cell apoptosis in experimental group was significantly higher than that of blank group and control group（ F = 48. 961,P 〈 0. 05）,48 h,72 h,96 h,120 h,SW480 cell proliferation in the experimental group were significantly lower than that of the control group and the control group（ P 〈 0. 05）,experimental group SPrdx2,Survivin mRNA and protein expression levels were significantly lower than those of control group and the control group（ P 〈 0. 05）. Transwell invasion assay showed that the experimental group transfected colorectal cancer SW480 cell invasion ability in blank group and control group（ P 〈 0. 05）. Conclusion Prdx2 gene silencing can inhibit the proliferation and promote apoptosis of colorectal cancer SW480 cells,but it can enhance the invasion ability of SW480 cells in colorectal cancer.