姜黄素对沙漠干热环境下大鼠肠黏膜损伤及氧化应激的影响

Protective effect of curcumin on the intestinal injury and oxidative stress in rats in dry and hot desert environment

目的探讨姜黄素预处理对沙漠干热环境下大鼠肠黏膜的保护作用。方法雄性SPF级大鼠80只,随机分为生理盐水对照组(NC组)40只、姜黄素预处理组(HDC组)40只。NC组生理盐水灌胃,HDC组200mg/kg姜黄素灌胃,连续7 d,每天1次。将各组大鼠置于模拟沙漠干热环境的实验舱中,温度:(41±0.5)℃,相对湿度:(10±2)%。在0、50、100、150 min时间点,分别随机取出NC组、HDC组大鼠(每组10只),腹腔注射戊巴比妥钠麻醉,留取回肠样本进行病理学观察评分及氧化应激指标CAT、SOD、MDA的检测。结果在0、50 min时间点,HDC组较NC组病理损伤评分无显著性差异(P>0.05);在100、150 min时间点,HDC组病理损伤评分较NC组显著降低(P<0.01)。在50、100、150 min时间点,HDC组CAT、SOD活力较NC组显著升高(P<0.05或P<0.01),HDC组MDA含量较NC组显著降低(P<0.05或P<0.01)。暴露于干热环境中,NC组肠黏膜病理损伤评分与CAT、SOD活力呈负相关(r=-0.9128,r=-0.9125,均P<0.01),与MDA含量呈正相关(r=0.9258,P<0.01)。结论姜黄素预处理对沙漠干热环境下大鼠肠黏膜具有保护作用,其机制可能与姜黄素抑制肠黏膜氧化应激反应有关。

Objective To investigate the protective effect of curcumin pretreatment on the intestinal mucosa in rats in dry and hot desert environment. Methods Eighty male SPF grade rats were randomly divided into saline control group（ NC group） and curcumin pretreatment group（ HDC group）（ 40 in each group）. Rats in the NC group were gavaged with saline,and rats in the HDC group were gavaged with curcumin（ 200 mg/kg）,once a day for 7 days. The ratswere placed in a cabin simulating the dry and hot desert environment（ 41 ± 0. 5） ℃ and relative humidity of（ 10 ± 2） %.Rats were randomly taken from each groups（ n = 10） and sacrificed with 3% sodium pentobarbital intraperitoneally at 0 min,50 min,100 min and 150 min time points. The ileal tissue was stained for histological examination and oxidative stress index was detected. Results At time points 0 min and 50 min,the pathological injury scores of the HDC group were not significantly different compared with the NC group（ P〈0. 05）. At time points 100 min and 150 min,the pathological injury scores of the HDC group were significantly decreased compared with the NC group（ P〈0. 01）. At time points50 min,100 min and 150 min,the CAT and SOD activity of HDC group were significantly increased compared with the NC group（ P〈0. 05 or P〈0. 01）. The MDA content of HDC group were significantly decreased compared with the NC group（ P〈0. 05 or P〈0. 01）. Exposed to dry and hot environment,the pathological injury scores of the NC group were negatively correlated with CAT and SOD activity（ r =-0. 9128,r =-0. 9125,P〈0. 01）,and positively correlated with MDA content（ r = 0. 9258,P〈0. 01）. Conclusions Curcumin pretreatment can protect the intestinal mucosa of rats in dry-heat environment of desert,and curcumin can alleviate the pathological damages of intestinal mucosa by inhibiting the oxidative stress in intestinal mucosa.