燕麦β-葡聚糖对脓毒症小肠损伤相关基因表达的影响

Effects of oat β-glucan on the expression of small intestine injury-related genes in septic rats

目的探索燕麦β-葡聚糖对脓毒症小肠损伤相关基因表达的影响。方法 SD大鼠随机分为正常对照组(NC组),实验对照组(TC组),燕麦β-葡聚糖组(Oglu组)。TC组和Oglu组以盲肠结扎穿孔(cecal ligation and puncture,CLP)法建立脓毒症模型。Oglu组以不同剂量燕麦β-葡聚糖灌胃。12 h后收集各组小肠组织,Western blot检测小肠组织损伤基因表达。结果 1)燕麦β-葡聚糖减轻脓毒症诱导的小肠损伤,降低脓毒症大鼠小肠组织中TNF-α、IL-1β及IL-6水平(P<0.05);(2)脓毒症组小肠损伤相关蛋白Bcl-2表达下调,Bax、caspase-3、Toll样、凋亡基因FAS和FASL、NF-κB表达明显增加;(3)燕麦β-葡聚糖组小肠粘膜Bax、caspase-3、Toll样、凋亡基因FAS和FASL、NF-κB的表达低于明显脓毒症组,而Bcl-2表达较高。结论燕麦β-葡聚糖调控脓毒症大鼠小肠损伤基因的表达,保护小肠上皮免受脓毒症的损伤。

Objective To explore the effect of oat β-glucan on gene expression in small intestine injury in sepsis.Methods SD rats were randomly divided into normal control group（ NC group）,experimental control group（ TC group）and oat β-glucan group（ Oglu group）. TC group and Oglu group were used to establish sepsis model with CLP method,and oglu group with different doses of oat β-glucan. After 12 h the small intestine tissue were collected. Western blot was used to detect gene expression in the small intestine tissue injury. Results 1） Oat β-glucan reduced sepsis-induced small intestine injury,and reduced the TNF-α,IL-1β and IL-6 levels in small intestine of septic rats.（ 2） The expression of small intestinal injury-related proteins such as Bax,caspase-3,Toll-like,apoptotic genes FAS and FASL and NF-κB were significantly increased,and Bcl-2 expression was down-regulated in the TC group.（ 3） The expressions of Bax,caspase-3,Toll-like,apoptotic genes FAS and FASL and NF-κB in oat β-glucan-treated rats were lower than that in the septic rats,while Bcl-2 expression was higher than the TC group. Conclusions Oat β-glucan regulates the expression of small intestinal injury genes in septic rats and protects the small intestinal epithelium against sepsis-induced injury.