中介素与肾上腺髓质素对局灶性脑缺血大鼠脑微循环的影响

Promoting effect of intermedin and adrenomedullin on cerebral microcirculation in rat models of cerebral ischemia

目的探讨中介素(intermedin,IMD)与肾上腺髓质素(adrenomedullin,ADM)对局灶性脑缺血大鼠脑微循环的影响。方法采用大脑中动脉闭塞法构建脑缺血(cerebral ischemia,CI)大鼠模型,将40只SPF级雄性SD大鼠随机分为3组:脑缺血模型+生理盐水[CI+NS(normal saline,NS)]组、CI模型+ADM处理(CI+ADM)组与CI模型+IMD处理(CI+IMD)组,采用激光多普勒测定仪监测脑表面微循环灌注量的变化。结果 CI+NS组、CI+ADM组与CI+IMD组大鼠脑表面微循环灌注量的比较,差异有显著性(P<0.05),事后多重比较发现,CI+IMD组大鼠脑表面微循环灌注量均高于CI+NS组与CI+ADM组。结论 IMD可以改善局灶性脑缺血大鼠脑微循环,且疗效优于ADM。

Objective To explore the effect of intermedin（ IMD） and adrenomedullin（ ADM） on cerebralmicrocirculation in rats with cerebral ischemia. Methods Rat cerebral ischemia（ CI） model was established by middle cerebral artery occlusion. 40 SPF male adult Sprague-Dawley（ SD） rats were randomly divided into three groups： CI ＋ NS（ normal saline） group,CI ＋ ADM group and CI ＋ IMD group,which were used to observe the changes of brain surface microcirculatory perfusion with a laser Doppler flowmeter. Results The differences of brain surface microcirculatory perfusion were statistically significant among the CI ＋ NS group,CI ＋ ADM group and CI ＋ IMD group（ F = 53. 426,P 0. 05）. Multiple comparison showed that the brain surface microcirculatory perfusion in the CI ＋ IMD group was higher than that of the CI ＋ NS group and CI ＋ ADM group. Conclusions Intermedin can improve the cerebral microcirculation in rats with cerebral ischemia,and its therapeutic effect is better than adrenomedullin.