山沉香超微粉与普通粉HPLC指纹图谱的比较研究

Comparative study on HPLC fingerprint of common powder and ultrafine powder of Syringa pinnatifolia

目的建立山沉香药材的HPLC指纹图谱,比较山沉香普通粉与超微粉指纹图谱的差异。方法采用Di KMA-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸水溶液,梯度洗脱,流速1.0 m L·min^(-1),检测波长281 nm,柱温25℃,进样量20μL。结果确立了29个山沉香药材指纹图谱的共有峰,10批山沉香药材的相似度评价结果均>0.9;山沉香经超微粉碎后,化学成分的种类及数目并未发生明显改变,但其主要化学成分的含量明显提高。结论所用方法准确可靠、重复性好,可作为山沉香药材质量控制的有效方法;超微粉碎能明显促进山沉香化学成分的溶出。

OBJECTIVE To establish an HPLC fingerprint and compare the differences between common powder and ultrafine powder of Syringa pinnatifolia Hemsl. METHODS The analysis was carried out on a DiKMA - C18 （ 250 mm ×4.6 mm, 5 μm ） column with gradient elution of methanol -0.4% phosphoric acid, the flow rate of 1.0 mL. min-1. The wavelength was 281 nm, The column temperature was 25℃ and the injection volume was 20μL. RESULTS Through the analysis of 10 batches of S. pinnatifolia. 29 mutual peaks were indicate, and the similarity of 10 batches of samples was greater than 0.90. Ultramicro comminution did not change number of main chemical composition, but it could greatly increase the content of main chemical composition. CONCLUSION The method of HPLC fingerprint is accurate, reliable and repeatable, which can be used for analyzing chemical constituents. Ultramicro comminution of S. pinnatifolia can obviously promote chemical dissolution.