转基因玉米和转基因大豆盲样检测方法

Determining Blind Samples of Transgenic Maize and Transgenic Soybean

转基因作物商业化发展迅速,中国每年进口大量转基因产品并高度重视我国转基因作物研发,制定了转基因作物及其产品的监管制度。现实迫切需要一套简单、可靠的盲样转基因成分检测方法。该研究以转基因大豆盲样(W160982和W160984)和转基因玉米盲样(W160983和W160985)为研究材料,根据农业部的转基因检测标准,采用普通PCR技术对作物中转基因成分进行检测。通过与标准样品比对并测序,最终确定盲样的转基因成分,玉米盲样W160983转基因成分与标准品MON89034一致,W160985与TC1507一致;大豆盲样W160984与BPS-CV127-9一致,W160982与GTS40-3-2一致。该研究提供并验证了一套玉米和大豆转基因盲样检测的方案,在转基因作物研究生产、进出境检测、监管等方面具有实际应用价值。

Genetically modified crops developed rapidly in the world. China imports a large amount of products of genetically modified crops every year, and has put billions of money in genetically modified crops research. China also has established regulations for genetically modified crops and their products. Simple and reliable methods for detecting transgenic ingredients are very urgent in China. In this study, according to the detection protocols of genetically modified crops published by the Ministry of Agriculture of the People＇s Republic of China, blind samples of soybean （Wl60982 and W160984） and maize （W160983 and W160985） were detected for transgenic ingredients using the PCR technology. As a result, W160983, W160985, W160982, and W160984 are the standard sample MON89034, TC1507, GTS40-3-2, and BPS-CV127-9, respectively. The results of the blind samples were validated by sequence alignment of the PCR product. This study provided an experimental protocol for the detection of transgenic maize and soybean.